| 李敏,戴二黑,戴瑞霞,周冬生,杨晓艳,崔百忠,金丽霞,赵海红,李存香,祁美英,次仁顿珠,戴翔,唐永娇,杨瑞馥.青藏高原鼠疫耶尔森菌基因型分布[J].中华流行病学杂志,2006,27(5):412-415 |
| 青藏高原鼠疫耶尔森菌基因型分布 |
| Study on the genotyping and microevolation of Yersinia Pestis in theQingbai-Tibet Plateau |
| 收稿日期:2005-08-25 出版日期:2014-09-12 |
| DOI： |
| 中文关键词: 鼠疫耶尔森菌 基因分型 差异区段 青藏高原 |
| 英文关键词: Yersinia pesos Genotyping Different regions Qinghai-Tibet Plateau |
| 基金项目: |
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| 中文摘要: |
| 目的研究青藏高原鼠疫耶尔森菌(鼠疫菌)基因组型分布特征。方法对分离到的青藏高原鼠疫菌297株,根据已经证实的22个差异区段设计引物,每株鼠疫菌的每个基因差异区段都采用PCR技术进行验证。结果在喜马拉雅旱獭鼠疫自然疫源地中,鼠疫菌基因组型有9种,分别为1、5、6、7、8、10、11、新基因组型和Ype-ancestor型,其中以5、8和10型为主,3种基因组型合计所占比例为80．6％(204／253),而且不同地区鼠疫菌基因组型的分布也不一致。青藏高原青海田鼠鼠疫疫源地鼠疫菌基因组型全部为14型。结论青藏高原鼠疫菌基因组型分布具有明显的地理特征。根据基因组型的分布状况推测出了鼠疫菌在青藏高原的传播路径。 |
| 英文摘要: |
| Objective To study the distribution of genomovars and microevolution of Yersinia pestis in the Qinghai-Tibet Plateau. Methods Primer pairs targeting the twenty-two different regions(DFRs)were designed for detecting the presence or deletion of each DFR in 297 strains isolated from the Qinghai-Tibet Plateau. Results 9 genomovars, i. e. Genomovar 1，5，6，7, 8，10，11，new type and Ype-ancestor were identified in the Marmots himalaynna plague focus of the Qinghai-Tibet Plateau. Among these genomovars,genomovar 5,8 and 10 were dominant types. The total rate of the three genomovars was $0.696 (204/253) and the genomovars in different regions were different. All of 44 strains of Y. pesos in the Microtus fuscus plague focus of the Qinghai-Tibet Plateau belonged to genomovar 14. Conclusion The distribution of genomovars of Y. pestis in the Qinghai-Tibet plateau had remarkable characteristics geographically. Based on the distribution of genomovars of Y. pestis，the routes of transmission and microevolution of Y. pestis were proposed. |
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