| 任淑华,倪晓平,孙建荣,高炎,项海青,孔庆鑫,赵洪峰,周田美,查捷,李华铭.社区获得性胃肠炎患者中分离出香港海鸥形菌的研究报告[J].中华流行病学杂志,2006,27(7):614-617 |
| 社区获得性胃肠炎患者中分离出香港海鸥形菌的研究报告 |
| A report on Laribacter hongkongensis isolated from a patient with community-acquired gastroenteritis |
| 收稿日期:2006-03-20 出版日期:2014-09-12 |
| DOI： |
| 中文关键词: 社区获得性胃肠炎 香港海鸥形菌 16S rRNA基因 药敏试验 |
| 英文关键词: Community-acquired gastroenteritis Laribacter hongkongensis 16S rRNA gene Drug susceptibility test |
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| 中文摘要: |
| 目的了解中国内地社区获得性胃肠炎患者是否存在香港海鸥形菌感染。方法于2005年8月对杭州市第一人民医院肠道门诊就医患者采集粪样,分别接种SS、XLD、CCDA、TCBS、MacConkey琼脂(MA)以及改良头孢哌酮MacConkey琼脂((2MA)等6种平皿,对分离到的可疑细菌进行常规生理生化实验、16S rRNA基因序列测定、透射电镜(TEM)下形态观察及药敏试验。结果编号为242的标本,在CMA平皿上生长出无色、透明的微小菌落。该菌为革兰阴性,氧化酶、过氧化氢酶、尿素酶和精氨酸双脱氢酶阳性,鸟氨酸脱羧酶和赖氨酸脱羧酶阴性,不发酵葡萄糖。经16S rRNA基因序列测定,菌株242的基因序列长度为1413 bp,采用:Blast软件对测序结果进行同源性分析显示,与文献报道的香港海鸥形菌同源值达100％。TEM下可见菌体两端有多根鞭毛。药敏试验结果显示,该菌对临床常用的21种抗菌药物表现出不同程度的耐药性,其中对青霉素、氨苄西林、头孢哌酮、头孢他啶表现为耐药。结论证实中国内地社区获得性胃肠炎患者粪便中存在香港海鸥形菌。有关该菌引起感染性腹泻疾病的致病地位与流行病学等特征需进一步深入研究。 |
| 英文摘要: |
| Objective To study the possibility of the presence of Laribacter hongkongensis in the community-acquired gastroenteritis in mainland China and to provide evidence for clinical diagnosis of this disease. Methods All fecal swabs were freshly collected from outpatients with community-acquired diarrhea in our hospital and immediately plated onto six kinds of culture agars: XLD,TCBS,SS, CCDA, MacConkey agar(MA) and modified cefoperazone MacConkey agar (CMA). Phenotypic characteristics, 16S rRNA gene sequencing, transmission electronmicroscopy (TEM)and drug susceptibility tests were employed to identify the isolated bacteria. Results Tiny,colourless, trans colonies growing on CMA of No. 242 sample were found. The colonies were composed of gram-negative bacterium which presented positive to oxidase, arginine dihydrolase, catalase and urease, and negative for lysine decarboxylase and ornithine decarboxylase. Glucose could not be fermented by this bacterium. PCR of the 16S rRNA gene of this bacterium showed fragment with 1413 bp in size. The 16S rRNA gene sequence of this bacterium was analyzed by Blast for a homology search, and the result showing that it had a 100% homology with Laribacter hongkongensis. Bipolar tuft flagella were observed by TEM. Drug susceptibility test indicated that this bacterium was resistant to 21 antibiotics at a variety of levels and definitely resistant to penicillin, ampicillin, cefoperazone and ceftazidime. Conclusion Data of our research proved the presence of Laribacter hongkongensis in the feces of patients with community-acquired gastroenteritis in mainland China. Further study was required on the clinical and epidemioiogical characteristics of the disease caused by this bacterium. |
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