| 王呜,李孝权,莫自耀,刘于飞,邓志爱,张欣强,沈纪川,张颖.脉冲场凝胶电泳方法在霍乱暴发溯源中的应用[J].中华流行病学杂志,2007,28(1):61-64 |
| 脉冲场凝胶电泳方法在霍乱暴发溯源中的应用 |
| Application of pulse-field gel electrophoresis analysis in the source-tracking of cholera epidemics |
| 收稿日期:2006-07-26 出版日期:2014-09-15 |
| DOI： |
| 中文关键词: 霍乱弧菌 脉冲场凝胶电泳 分子分型 噬菌体一生物分型 |
| 英文关键词: Vibrio cholerae Pulse-field gel electrophoresis analysis Molecular subtyping Phage-biological typing |
| 基金项目:广东省医学科学技术研究基金(A2002059)；广州市科技计划(2005儿一c0191) |
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| 中文摘要: |
| 目的应用脉冲场凝胶电泳(PFGE)方法对不同类型的霍乱暴发事件进行分析，了解霍乱菌株的PFGE特征，追溯菌株的来源及其演变过程，探讨霍乱暴发流行的特点和规律。方法采用限制性内切酶NofI，对2003—2005年广州地区霍乱暴发疫情中41株霍乱弧菌进行PFGE分子分型．用BioNumericsversion4．0软件(复选Dice相关系数和uPGMA方法)进行聚类分析，并与噬菌体一生物分型及致病相关基因分型方法作比较。结果在霍乱暴发事件中，PFGE能有效地区分流行病学上密切相关菌株与无关菌株，与噬菌体．生物分型和致病相关基因分型方法相比具有更高的分辨能力。结论PFGE分型可揭示人和环境分离的霍乱菌株之间的流行病学联系，为霍乱疫情溯源提供分子流行病学证据和支持。 |
| 英文摘要: |
| Objective To apply pulse-field gel electrophoresis analysis(PFGE) in the analysis ofcholera outbreak events and to determine the molecular epidemiological characteristics of Vibrio cholerae(V. cholerae)isolates. Methods PFGE using restriction enzyme Not I was employed in the molecularsubtyping of forty-one strains of V. cholerae isolated in cholera outbreak events from 2003 to 2005 inGuangzhou area and PFGE patterns were analyzed by BioNumerics Version 4.0 software to perform clusteranalysis. Pattern profiles were compared by utilizing of Dice coefficient and UPGMA(unweighted pair groupmethod with arithmetic averages), Comparison of PFGE typing Results was performed with phage-biologicaltyping and pathogenicity-associated genes typing. Results In cholera outbreak events, PFGE coulddiscriminate epidemiologically related and unrelated strains, having more discriminatory power than phage-biological typing and pathogenicity-associated genes-typing. Conclusions Molecular sub-typing by PFGEcould disclose the epidemiological relationships of strains from humans and the environment, providingmolecular epidemiological evidence and support for the source-tracking of cholera outbreak events. |
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