| 田炳均,吴燕,张东华,何丽芳,丁峥嵘,陆林.云南省1997-2000年及2004年非脊髓灰质炎肠道病毒分子生物学及流行特征分析[J].中华流行病学杂志,2007,28(4):346-349 |
| 云南省1997-2000年及2004年非脊髓灰质炎肠道病毒分子生物学及流行特征分析 |
| Study on the molecular typing and epidemiology of non-polio enteroviruses isolated from Yunnan province, China |
| 收稿日期:2006-10-25 出版日期:2014-09-12 |
| DOI： |
| 中文关键词: 非脊髓灰质炎肠道病毒 分子生物学定型 流行病学 |
| 英文关键词: Non—polio enteroviruses Molecular typing Epidemiology |
| 基金项目: |
| 作者 | 单位 | | 田炳均 | 650022, 昆明, 云南省疾病预防控制中心 | | 吴燕 | 650022, 昆明, 云南省疾病预防控制中心 | | 张东华 | 650022, 昆明, 云南省疾病预防控制中心 | | 何丽芳 | 650022, 昆明, 云南省疾病预防控制中心 | | 丁峥嵘 | 650022, 昆明, 云南省疾病预防控制中心 | | 陆林 | 650022, 昆明, 云南省疾病预防控制中心 |
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| 中文摘要: |
| 目的<\b> 描述云南省1997-2000年及2004年5年的肠道病毒(EV)分离状况及型别特征.方法<\b> 5年在急性迟缓性麻痹病例中共检测到210株非脊髓灰质炎(脊灰)肠道病毒(NPEVs),其中12株经血清学鉴定为腺病毒.剩余198株经基因测序定型,即病毒VP1区基因序列转变为氨基酸序列后与标准株进行比较.结果<\b> 经分子生物学定型:人类EV-A组5株(5个血清型)、EV-B组158株(34个血清型)、EV-C组32株(6个血清型)、未分离到EV-D组病毒.在急性迟缓性麻痹病例监测中,210个分离株,EV-B组病毒占75.2%,为主要型别;EV-C组占12.2%,腺病毒占5.7%,EV-A组占2.4%.结论<\b> 云南省EV的流行中,以EV-B组较多. |
| 英文摘要: |
| Objective<\b> This report presented an overview on the epidemiology of enterovirus in Yunnan province，the People’s Republic of China.Methods<\b> A total of 210 strains of non-polioviruses isolated under acute flaccid paralysis surveillance during a 5-year study period from 1997 to 2000 and 2004 were examined.0f the 210 non-polioviruses strains，a total of 12 strains of aderloviruses were serologically identified.The remaining 198 isolates were used for molecular typing，and the viral genomes of 195 non- polio enteroviruses(NPEVs) were translated to corresponding amillo acid sequences and compared with those of the prototype strains.ResuIts Based on m。lecular typing， 5 isolates were classified int。5 serotypes of human enterovirus A species while 158 isolates into 34 serotypes of B and 32 isOlates into 6 serotypes of C species.However， we did not isolate any viruses which belonged to human enterovirus D species.Thus，under acute flaccid paralvsis surveillance，human enterovirus B species accounted for 75.2％ of the 210 isolates and was considered as the predominant one，followed by human enterovirus C(12.2％)， adenovjrus(5，7％)， and human enterovirus A(2，4％).ConcIusi彻Although the epidemiological characte“stics of NPEVs from Yunnan province remained“unkllown”， the molecular typing method had provided us a breakthrough to undcrstand the epidemiology of these viruses. |
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