田炳均,吴燕,张东华,何丽芳,丁峥嵘,陆林.云南省1997-2000年及2004年非脊髓灰质炎肠道病毒分子生物学及流行特征分析[J].中华流行病学杂志,2007,28(4):346-349 |
云南省1997-2000年及2004年非脊髓灰质炎肠道病毒分子生物学及流行特征分析 |
Study on the molecular typing and epidemiology of non-polio enteroviruses isolated from Yunnan province, China |
收稿日期:2006-10-25 出版日期:2014-09-12 |
DOI: |
中文关键词: 非脊髓灰质炎肠道病毒 分子生物学定型 流行病学 |
英文关键词: Non—polio enteroviruses Molecular typing Epidemiology |
基金项目: |
作者 | 单位 | 田炳均 | 650022, 昆明, 云南省疾病预防控制中心 | 吴燕 | 650022, 昆明, 云南省疾病预防控制中心 | 张东华 | 650022, 昆明, 云南省疾病预防控制中心 | 何丽芳 | 650022, 昆明, 云南省疾病预防控制中心 | 丁峥嵘 | 650022, 昆明, 云南省疾病预防控制中心 | 陆林 | 650022, 昆明, 云南省疾病预防控制中心 |
|
摘要点击次数: 3008 |
全文下载次数: 1249 |
中文摘要: |
目的<\b> 描述云南省1997-2000年及2004年5年的肠道病毒(EV)分离状况及型别特征.方法<\b> 5年在急性迟缓性麻痹病例中共检测到210株非脊髓灰质炎(脊灰)肠道病毒(NPEVs),其中12株经血清学鉴定为腺病毒.剩余198株经基因测序定型,即病毒VP1区基因序列转变为氨基酸序列后与标准株进行比较.结果<\b> 经分子生物学定型:人类EV-A组5株(5个血清型)、EV-B组158株(34个血清型)、EV-C组32株(6个血清型)、未分离到EV-D组病毒.在急性迟缓性麻痹病例监测中,210个分离株,EV-B组病毒占75.2%,为主要型别;EV-C组占12.2%,腺病毒占5.7%,EV-A组占2.4%.结论<\b> 云南省EV的流行中,以EV-B组较多. |
英文摘要: |
Objective<\b> This report presented an overview on the epidemiology of enterovirus in Yunnan province,the People’s Republic of China.Methods<\b> A total of 210 strains of non-polioviruses isolated under acute flaccid paralysis surveillance during a 5-year study period from 1997 to 2000 and 2004 were examined.0f the 210 non-polioviruses strains,a total of 12 strains of aderloviruses were serologically identified.The remaining 198 isolates were used for molecular typing,and the viral genomes of 195 non- polio enteroviruses(NPEVs) were translated to corresponding amillo acid sequences and compared with those of the prototype strains.ResuIts Based on m。lecular typing, 5 isolates were classified int。5 serotypes of human enterovirus A species while 158 isolates into 34 serotypes of B and 32 isOlates into 6 serotypes of C species.However, we did not isolate any viruses which belonged to human enterovirus D species.Thus,under acute flaccid paralvsis surveillance,human enterovirus B species accounted for 75.2% of the 210 isolates and was considered as the predominant one,followed by human enterovirus C(12.2%), adenovjrus(5,7%), and human enterovirus A(2,4%).ConcIusi彻Although the epidemiological characte“stics of NPEVs from Yunnan province remained“unkllown”, the molecular typing method had provided us a breakthrough to undcrstand the epidemiology of these viruses. |
查看全文
Html全文
查看/发表评论 下载PDF阅读器 |
|
关闭 |
|
|
|