| 叶荣荣,邢辉,刘海林,黑发欣,赵月娟,刘胜牙,孙伟东,张启云,张琴,卢红艳,贺雄,邵一鸣.北京市2006年HIV一1流行毒株的gag和env基因序列测定及亚型分析[J].中华流行病学杂志,2007,28(6):586-588 |
| 北京市2006年HIV一1流行毒株的gag和env基因序列测定及亚型分析 |
| Subtyped sequence anaIysis of gag and enV genes among HIV-1 strains circulating in Beijing residents during 2006 |
| 收稿日期: 出版日期:2014-09-12 |
| DOI： |
| 中文关键词: 人类免疫缺陷病毒1型 亚型 巢式聚合酶链反应 |
| 英文关键词: Human Imunodeficiency virus-1 Subtype Nest-polymerase chain reaction |
| 基金项目:国家高科技发展(863)计划资助项目(2006AA022418) |
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| 中文摘要: |
| 目的监测2006年北京市北京户籍HIV感染者中HIV-l型流行病毒株的亚型分布特点和变化规律。方法采集北京市2006年新确认北京户籍HIV-l感染者的抗凝全血样品32份, 分离血浆, 提取病毒RNA, 用巢式聚合酶链反应方法扩增病毒gag和enu基因, 并进行序列测定和亚型分析。结果系统进化分析表明北京户籍HIV一1感染者样品分别属于5个亚型, 分别为B亚型9份, 泰国B亚型2份、C亚型2份, 流行重组型CRF07BC亚型5份, 流行重组型CRF01AE亚型4份。结论北京市居民中已存在多种HIv．1亚型和流行重组型, 应加强对HIv-1毒株亚型变异的监测, 及时调整防治策略。 |
| 英文摘要: |
| 0bjectiveTo investigate the subtype distribution and the prevalence of sequence characteristics of H11-1 strains in IBeijing residents during 2006 and to analyze the relationship between distribution of HIV一1 subtypes and transmission mutines.MethodsBlood samples from 32 new confirmed H1V-1 infected individuals from Beijing residents in 2006 and separated plasma specimens were collected.RNAs were extracted and the gag and env gene were amplified by RT-PCR and nest-PCR.PCR products were sequenced directly and phylogenetic analyses of gag and env gene were performed using the MEGA2 software.ResultsAmong 32 HIV一1 plasma samples, 22 gag and env gene fragments were amplified and analyzed.Five HIV一1 subtypes or circulating recombinant forms(CRFs)of HIV一1 including Thai B(2strains), B(9 strajns), C(2 strains), CRF07 BC(5 strains), CRF01 AE(4 strains)were identified being Circulated in Beijing.The gene divergences of gag gene inside the subtypes were 6.6%, 4.3%, 6.8%, 4.9%and 3.O%in subtype B, Thai B, C, CRF01 AE and CRF07 BC respectively.Subtypes B were predominant in Beijing, accounted for 40.9%among 22 samples.ConclusionFive HIV一1 subtypes were identified in Beijing and the surveillance of HIV一1 gene variation should be paid more attention to. |
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