| 孙肖红,王环宇,潘晓玲,付士红,冯云,孟维珊,梁国栋.中国滇西北地区分离流行性乙型脑炎病毒的分子特征研[J].中华流行病学杂志,2009,30(6):606-610 |
| 中国滇西北地区分离流行性乙型脑炎病毒的分子特征研 |
| Analysis on the molecular characteristics of Japanese encephalitis virus isolated in Northwestern Yunnan province |
| 收稿日期:2009-01-16 出版日期:2014-09-12 |
| DOI： |
| 中文关键词: 流行性乙型脑炎病毒 分子特征 基因分型 |
| 英文关键词: Japanese encephalitis virus Molecular characteristics Genotype |
| 基金项目:科技部重大传染病专项(2003BA712A08-01);中美合作项目(U19-GH000004) |
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| 中文摘要: |
| 目的 对中国滇西北地区分离的流行性乙型脑炎(乙脑)病毒进行分子特征研究,了解与云南省内其他地区病毒株的差异.方法 用RT-PCR的方法扩增滇西北地区分离的13株乙脑病毒PrM、E片段和3'非编码区,对扩增产物进行序列测定.用Clustal 1.8X、DNASTAR、GENEDOC等生物学软件进行核苷酸序列和系统进化分析.结果 基于PrM和E基因核苷酸序列的系统进化显示,滇西北分离的13株乙脑病毒中有12株属于基因Ⅰ型,1株为基因Ⅲ型;12株基因Ⅰ型乙脑病毒与云南省其他地区分离的病毒株处于不同分支;和云南省其他地区分离株之间E基因核苷酸序列差异度为0.2%～13.9%;3'非编码区存在两种核苷酸缺失情况,基因Ⅰ型毒株在终止密码子后出现3处缺失,而基因Ⅲ型毒株只有1处缺失.结论 滇西北地区乙脑病毒分离株以基因Ⅰ型为主,E基因核苷酸序列与云南省其他地区分离株差异不明显;基因Ⅰ型毒株在3'非编码区存在3处缺失,而基因Ⅲ型毒株则只有1处缺失. |
| 英文摘要: |
| Objective To analyze the molecular characteristics of Japanese encephalitis virus (JEV) isolated in Northwestern Yunnan province, and to clarify the differences between the strains isolated in Northwestern and other parts of Yunnan province. Methods PrM, E and 3' untranslated region nueleotide acid sequences of the isolates were amplified by RT-PCR and then sequenced. Sequence alignment and phylogenetic analysis were performed by using Clustal 1.8X, DNASTAR, GENEDOC and Mega 3.1 programs. Results 12 of the 13 isolates of JEV obtained in Northwestern Yunnan were identified as genotype Ⅰ, only one strain was genotype Ⅲ of JEV. The 12 strains of genotype Ⅰ were clustered in different branches with other isolates obtained in other parts of Yunnan province. Data from sequence analysis on E gene found that the nucleotide identity was 0.2%-13.9% between the Northwestern isolates and other Yunnan strains. There were two kinds of nucleotides deletion patterns at 3' untranslated region with three and one deletions was found after termination eodon in genotype Ⅰ and Ⅲ isolates, respectively. Conclusion There were two genotypes of Ⅰ and Ⅲ in 13 strains of JEV in this study and genotype Ⅰ isolates were predominant (12/13). There were no apparent differences in E gene sequence between isolates obtained in the Northwestern and other parts of Yunnan. Three deletions were found in 3' untranslated region in genotypes Ⅰ isolates and one deletion was in genotypes Ⅲ. |
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