| 汪霞,徐葛林,吴杰,罗静,朱家鸿,周剑军,施南峰,唐青,张永振,唐建蓉,吴泰才,明贺田,沈蕊华,陈焕春.细胞培养法检测和分离狂犬病街毒的可行性研究[J].中华流行病学杂志,2009,30(6):611-615 |
| 细胞培养法检测和分离狂犬病街毒的可行性研究 |
| Study on the feasibility of a cell-culture inoculation test in the detection and isolation of street rabies virus |
| 收稿日期:2009-02-16 出版日期:2014-09-12 |
| DOI： |
| 中文关键词: 狂犬病街毒 细胞培养法 检测 |
| 英文关键词: Street rabies virus Cell-culture inoculation test Detection |
| 基金项目:广东省科技计划(20006B36008005);全军医药卫生科研基金(06MA129) |
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| 中文摘要: |
| 目的 研究MNA细胞培养法检测和分离狂犬病街毒的可行性.方法 用MNA细胞培养法、荧光抗体实验(FAT)和双抗体夹心ELISA方法分别检测33份狂犬病街毒样品、20份狂犬病毒阴性犬脑样品和4份正常鼠脑样品.结果 MNA细胞培养法检测57份待检样品,培养48 h的实验结果显示,33份狂犬病街毒样品均为阳性,狂犬病毒阴性犬脑样品和正常鼠脑样品均为阴性,与FAT、双抗体夹心ELISA方法的检测结果完全一致.结论 MNA细胞培养法对样品中狂犬病街毒的检测具有较高的灵敏度和特异性,可用于狂犬病街毒的检测和分离. |
| 英文摘要: |
| Objective Feasibility of using MNA cell-culture inoculation test to detect and isolate the street rabies virus. Methods Using MNA cell-culture inoculation test, fluorescent antibody test (FAT) and sandwich ELISA with double-antibodies to detect 33 specimens of street rabies virus, 20 specimens of negative canine brains and 4 specimens of healthy mice brains. Results 33 specimens of street rabies virus were positive to the cell-culture inoculation test but the others were negative. The concordances of MNA cell-cultured inoculation test with FAT and sandwich ELISA with double-antibodies were both 100%. Conclusion MNA cell-culture inoculation test appeared to be both highly sensitive and specific in detecting the street rabies virus, and could be used in detection and isolation of the virus. |
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