文章摘要
夏玉刚,陆一涵,胡安群,秦雪,董晓莲,朱建福,姜庆五,郑英杰.华东和华南地区商品猪戊型肝炎病毒携带率和基因分型分析[J].中华流行病学杂志,2010,31(7):791-794
华东和华南地区商品猪戊型肝炎病毒携带率和基因分型分析
Study on the prevalence and genotype of hepatitis E virus among commercial swine population in Eastern and Southern China
收稿日期:2010-01-11  出版日期:2014-09-10
DOI:
中文关键词: 戊型肝炎病毒  携带率  基因型  
英文关键词: Hepatitis E virus  Prevalence  Genotype  Swine
基金项目:国家自然科学基金(30771842,30872158);上海市重点学科项目(Bll8
作者单位E-mail
夏玉刚 复旦大学公共卫生学院流行病学教研室教育部公共卫生安全重点实验室, 上海 200032  
陆一涵 复旦大学公共卫生学院流行病学教研室教育部公共卫生安全重点实验室, 上海 200032  
胡安群 复旦大学公共卫生学院流行病学教研室教育部公共卫生安全重点实验室, 上海 200032  
秦雪 复旦大学公共卫生学院流行病学教研室教育部公共卫生安全重点实验室, 上海 200032  
董晓莲 安徽省安庆市立医院  
朱建福 广西医科大学第一附属医院  
姜庆五 浙江省德清县疾病预防控制中心  
郑英杰 浙江省德清县疾病预防控制中心 yjzheng@shmu.edu.cn 
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中文摘要:
      目的 了解华东和华南地区商品猪戊型肝炎病毒(HEV)的携带率和基因分型.方法 2007-2009年收集华东和华南地区5家屠宰场商品猪胆汁600份,应用巢式RT-PCR方法检测HEVRNA,测序后做系统进化分析.结果 600份猪胆汁中有47份(7.83%)分离出HEVRNA.基于HEV ORF2区域内150-nt序列的系统进化分析结果,分离的47份HEV病毒株均属于Ⅳ型,与Ⅰ、Ⅱ、Ⅲ和Ⅳ型参考病毒株的核苷酸同源性分别为75.0%~83.4%、75.0%~84.6%、71.9%~80.7%和88.1%~91.5%,且大部分病毒株按采样地区来源表现出地区聚集性.结论 HEV在华东和华南地区的商品猪中广泛存在,应加强对商品猪食用安全问题的关注.
英文摘要:
      Objective To determine the prevalence and genotype of hepatitis E virus (HEV)among commercial swine population in Eastern and Southern China.Methods Six hundred specimens of swine bile collected from 5 slaughterhouses in Eastern and Southern China from 2007 to 2009 were tested for HEV RNA using nested RT-PCR. PCR products were sequenced for phylogenetic analysis. Results Forty-seven out of the 600 samples (7.83%) were positive for HEV RNA. Based on the 150 nt fragment within HEV ORF2, data from phylogenetic analysis revealed that all the 47 HEV isolates were identified to be genotype Ⅳ, sharing 75.0%-83.4%, 75.0%-84.6%, 71.9%-80.7% and 88.1%-91.5% nucleotide identities with prototype Ⅰ,Ⅱ,Ⅲ and Ⅳ HEV strains respectively while majority of the isolates clustered within their respective isolation sites. Conclusion HEV was widespread in commercial swine population in Eastern and Southern China that raised a serious concern about the safety regarding the consumption of pork products.
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