文章摘要
傅见英,姜葵,张茂俊,何利华,张建中.幽门螺杆菌中国分离株vacA基因多态性分析[J].中华流行病学杂志,2011,32(6):602-607
幽门螺杆菌中国分离株vacA基因多态性分析
Polymorphism of Helicobacter pylori vacA, isolated in China
收稿日期:2011-02-11  出版日期:2014-09-10
DOI:
中文关键词: 幽门螺杆菌;vacA等位基因多态性;分子流行病学
英文关键词: Helicobacter pylori;vacA allele polymorphism;Molecular epidemiology
基金项目:"十一五"国家科技支撑计划(2007BAD4802);国家科技重大专项(2008ZX10004-002)
作者单位E-mail
傅见英 天津医科大学总医院消化科, 300052
中国疾病预防控制中心传染病预防控制所诊断室,传染病预防控制国家重点实验室 
 
姜葵 天津医科大学总医院消化科, 300052  
张茂俊 中国疾病预防控制中心传染病预防控制所诊断室,传染病预防控制国家重点实验室  
何利华 中国疾病预防控制中心传染病预防控制所诊断室,传染病预防控制国家重点实验室  
张建中 中国疾病预防控制中心传染病预防控制所诊断室,传染病预防控制国家重点实验室 zhanjianzhong@icdc.cn 
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中文摘要:
      目的 分析幽门螺杆菌(HP)中国菌株vacA基因多态性.方法 对分离自中国7个不同地区、不同胃十二指肠相关疾病患者的119株HP,采用特异引物聚合酶链反应(PER)方法,对其vacA基因进行PCR扩增.根据核酸电泳中产物片段大小确定vacA等位基因类型并统计分析各型分布.对vacA基因核心片段进行PCR扩增和DNA测序,利用软件MEGA4.0对DNA测序结果进行聚类分析.结果 119株HP的vacA基因以sla、m2和il型为主,分别为97.5%(116/119)、68.9%(82/119)和91.6%(109/119).26.1%(31/119)为mlb;slb,mla未检出.vacA组合基因型以sla/m2/il为主(62.2%,74/119),sla/mlb/il次之(25.2%,30/119).不同地区、不同疾病来源菌株sla分布的差异无统计学意义(P>005).而m区基因多态性在疾病类型及分离地区间差异有统计学意义(P<0.01).不同疾病来源菌株间i区基因分型分布的差异无统计学意义,但不同分离地区菌株间差异有统计学意义(P<0.01).119株的vacA基因序列聚类为三个不同组群.结论 HP中国分离株vacA基因型以sla/m2/il组合型为主,sla分型结果与菌株分离地区及疾病类型无关.不同地区、不同疾病来源菌株m区基因分型不同.不同地区菌株vacA基因i区分型不同,但i区基因分型与菌株的疾病来源无显著相关性.
英文摘要:
      Objective To understand the polymorphism of Helicobacter pylori (H. pylori) vacA alleles in China. Methods A total of 119 H. pylori strains were isolated from different gastroduodenal diseases in 7 different geographic regions in China. vacA and its alleles were identified according to the length of PCR products with DNA electrophoresis. The distributions of vacA alleles were statistically analyzed. The core fragment of vacA was sequentially analyzed by software MEGA4.0. Results The alleles in vacA dominantly belonged to sla, m2 and il in the tested strains.The distribution appeared to be 97.5%(116/119),68.9%(82/119) and 91.6%(109/119),respectively.The mlb allele appeared to be 26.1% (31/119). slb and mla were not found. The major vacA recombination was between slaim2/il and 62.2%, followed by sla/mlb/il (25.2%, 30/119). No association was found between the distribution of sla allele and the clinical outcome, as well as the geographical regions (P>0.05). However, the distribution of m alleles showed significant difference both among the types of disease and the geographic regions (P<0.01), The present of i alleles did not show significant differences among disease patterns, but had significant differences between different geographic groups (P<0.01). Three clusters were identified among these 119 isolates according to the DNA sequence of vacA. Conclusion sla/m2/il appeared to be the main allele in H. pylori vacA isolates from China in this study. The distribution of m alleles in vacA was correlated both to the regions and the disease patterns. The presence of i allele was associated to the regions but not the disease patterns.
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