| 李韩平,韩扬,朱新鹏,路新利,郭伟,刘永健,鲍作义,李林,庄道民,刘思扬,李敬云.HIV-1N348I耐药突变流行状况及突变模式的研究[J].中华流行病学杂志,2011,32(9):908-912 |
| HIV-1N348I耐药突变流行状况及突变模式的研究 |
| Studying on the prevalence and mutation pattern of N348I which related to the resistance of HIV-1 |
| 收稿日期:2011-04-02 出版日期:2014-09-11 |
| DOI： |
| 中文关键词: 艾滋病毒1型 耐药 N348I |
| 英文关键词: HIV-1 Resistance N348I |
| 基金项目:国家自然科学基金（30800938,30830088）：国家“十一五”科技重大专项（2008ZX10001-004） |
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| 中文摘要: |
| 目的 阐明HIV-1耐药相关突变N348I在中国艾滋病患者中的流行率及突变模式。方法用RT-PCR从血浆标本中扩增614例治疗失败和619例未治疗的艾滋病患者的HIV-1 pol基因蛋白酶(PR)反转录酶(RT)全长基因(2100 bp)。将获得的1233条序列提交斯坦福大学HIV-1耐药数据库，根据数据库比对结果，分析N348I出现的频率及模式。结果N348I突变在抗病毒治疗失败患者中的流行率为6.5％,在未治疗患者中的流行率为0.8％。使用含齐多夫定(AZT)方案治疗的患者，N348I突变的流行率显著高于使用不含AZT方案治疗的患者(14.1％ vs.4.7％，x2=10.21,P＜0.01)；在出现N348I突变的接受治疗的患者中，N3481均以与其他突变联合的形式出现，与胸苷类似物耐药突变(TAMs)共同发生占85.0％(34/40)、与M184V/I共同出现占52.5％。结论使用一线方案治疗失败的艾滋病患者中，N348I突变有一定流行，该突变的发生以特有突变模式出现。 |
| 英文摘要: |
| Objective To elucidate the prevalence and the mutation pattern of N348I that related to the resistance seen in the AIDS patients, in China. Methods Partial pol gene of HIV-1 comprising of full protease (PR) and reverse transeriptase (RT) was obtained from plasma samples of therapy-failure individuals (n=614) and therapy-naive individuals (n=619) by using reverse transcription polymerase chain reaction(RT-PCR). 1233 sequences were then submitted to the HIV-1 drug resistance database of the Stanford University to analyze the prevalence and the emergence pattern of N348I.Results The prevalence of N348I was 6.5％ in the therapy-failure patients and 0.8％ in the naive individuals, respectively. The prevalence of N348I was more popular among those patients whose ART regimens containing zidovudine (AZT or ZDV) than those without AZT in regimens( 14.1％ vs. 4.7％, x2=10.21, P＜0.01 ). N3481 always emerged, and combined with others mutations among patients of ART, whose frequencies were: 85.0％ in combination with thymidine analog mutations (TAMs) and 52.5％ with M184V/I, respectively. Conclusion N348I was somehow prevalent in the therapy-failure patients when using the first-line antiretroviral drugs,and it emerged as unique patterns. This study laid the ground in improving the techaology of drug resistance genotypes detection and providing theoretical basis to study the mechanism of resistance and the law of molecular evolution. |
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