| 陈敏,马艳玲,楚承霞,邢辉,徐艳声,苏莹珍,杨颖,陈会超,罗红兵,贾曼红,陆林.昆明市2010年HIV-1耐药传播警戒线调查[J].中华流行病学杂志,2012,33(1):75-77 |
| 昆明市2010年HIV-1耐药传播警戒线调查 |
| Survey On the transmission of HⅣdrug resistance in Kunming，Yunnan province in 2010 |
| 收稿日期:2011-07-23 出版日期:2014-09-10 |
| DOI： |
| 中文关键词: 艾滋病病毒 耐药警戒线检测 抗病毒治疗 |
| 英文关键词: Human immunodeficiency virus Drug resistance transmission threshold survey Antiretroviral therapy |
| 基金项目:国家“十一五”科技重大专项(2009ZXl0004—902) |
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| 中文摘要: |
| 目的 了解2010年昆明市未经抗病毒治疗的HIV感染者中耐药株的传播水平。方法 根据WHO推荐的HIV耐药警戒线调查方法，对2010年1-8月收集的62份年龄＜25岁的HIV-1新发现感染者的血液样本进行HIV-1耐药基因型检测和耐药株传播水平分析。结果 62份样本中，54份完成耐药基因型鉴定。按pol基因区进行分型，其中53.2％的样本为CRF08 BC亚型，其他亚型包括CRF07_BC、CRF01_AE、C。根据采样时间顺序，前47份扩增阳性的样本用于耐药传播水平的分析，其中1份出现针对蛋白酶抑制剂类药物的耐药突变位点，耐药株流行率＜5％。结论 昆明市HIV耐药株尚处于低度流行水平，为控制耐药传播水平的上升，规范治疗及科学管理仍然是当前艾滋病患者治疗工作的重点。 |
| 英文摘要: |
| Objective To study the HIV drug resistance (HIVDR) transmission in Kunming city of Yunnan province in 2010.Methods Referring to the guidelines for HIV drug resistance threshold survey (HIVDR-TS) set by WHO,62 plasma samples of recently reported HIV-infected individuals who were older than 25 years of age,were collected from January to August 2010.Genotyping of pol genetic mutations associated with HIVDR with reverse transcriptional PCR was performed and the prevalence of HIV-1 drug resistance transmission was evaluated.Results Of the 62 plasma samples,54 were successfully sequenced and genotyped on pol sequence.Based on the pol sequences,HIV subtypes including CRF08_BC ( 53.2％ ),CRF07_BC ( 25.5％ ),CRF01_AE ( 19.1％ )and C (2.1％) were identified.According to the time of sampling,the first 47 sequenced samples were used for drug resistance prevalence analysis.A protease inhibitor (PI) relative mutation was found in one sample.Based on the WHO standard,the prevalence of transmitted HIV-1 drug resistance was ＜5％.Conclusion HIV-1 drug resistant strains transmission was still catalogued as low prevalence level in Kunming.To prevent the increase of HIVDR prevalence,normative treatment and scientific management to AIDS patients seemed to be quite important. |
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