文章摘要
张武林,张文东,赵焕云,齐绣花,胡挺松,吕粤,岳亮,邱薇,冯子良,郑颖,范泉水,张应国,贾杏林,张富强.云南省边境地区禽流感H5N1亚型病毒遗传多样性分析[J].中华流行病学杂志,2012,33(3):323-327
云南省边境地区禽流感H5N1亚型病毒遗传多样性分析
Genetic diversification of avian influenza H5N1 virus in boundary areas of Yunnan province
  
DOI:
中文关键词: 禽流感病毒;H5N1亚型;血凝素;遗传多样性
英文关键词: Avian influenza virus;H5N1 subtype;Hemagglutinin;Genetic diversification
基金项目:公益性行业(农业)科研专项(201103008);军队科技攻关项目(08G034);云南省科技计划项目
作者单位E-mail
张武林 湖南农业大学, 长沙 410128
成都军区疾病预防控制中心 
 
张文东 云南省动物疫病预防控制中心  
赵焕云 云南省动物疫病预防控制中心  
齐绣花 云南农业大学  
胡挺松 成都军区疾病预防控制中心  
吕粤 云南省动物疫病预防控制中心  
岳亮 云南省动物疫病预防控制中心  
邱薇 成都军区疾病预防控制中心  
冯子良 成都军区疾病预防控制中心  
郑颖 成都军区疾病预防控制中心  
范泉水 成都军区疾病预防控制中心  
张应国 云南省动物疫病预防控制中心  
贾杏林 湖南农业大学, 长沙 410128  
张富强 成都军区疾病预防控制中心 zfq1968@yahoo.com.cn 
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中文摘要:
      目的 了解云南省边境地区禽流感H5N1亚型病毒遗传多样性.方法 2009-2011年7月在云南省边境地区采集境外家禽和野生鸟类棉拭子样品,经H5/Nl亚型特异性多重RT-PCR检测,阳性样品进行病毒HA基因扩增,克隆至pMD 18-T载体测序,并与已知参考毒株进行序列比对及系统发育分析.结果 36份阳性样品病毒HA基因测序获得15种HA序列,存在2个不同进化分支(2.3.2、2.3.4),2.3.2进一步可划分为3个进化小分支(Ⅱ-1~Ⅱ-3),2.3.4进一步可划分为2个进化小分支(Ⅰ-1和Ⅰ-2).2.3.2Ⅱ-1、Ⅱ-2毒株是新出现的H5N1亚型病毒变异株.结论 2009- 2011年7月云南省边境地区H5N1亚型病毒具有遗传多样性,病毒经历了多分支(2.3.2、2.3.4)至单一支(2.3.2)的进化过程.
英文摘要:
      Objective To elucidate the genetic diversifications of avian influenza subtype H5N1 viruses in the boundary regions of Yunnan province during 2009 to July,2011.Methods Swab samples were collected from foreign poultry and wild birds in boundary regions of Yunnan province during 2009 to July,2011 and tested by H5/N1 subtype-specific multiplex RT-PCR.The HA genes of H5N1 virus from the positive samples were amplified by RT-PCR and cloned into pMD 18-T vectors for sequencing.Both alignment and phylogenetic analysis were performed with sequences of the known reference strains.Results Fifteen different HA sequences were obtained from 36 representative positive samples and could be divided into 2 distinct Clades (2.3.2 and 2.3.4).Through phylogenetic analysis,Clade 2.3.2 and 2.3.4 could then be further divided into 3 ( Ⅱ -1 to Ⅱ -3 ) and 2 smaller clades ( Ⅰ -1 and Ⅰ -2),respectively.The viruses of Clade 2.3.2 Ⅱ -1 and Ⅱ-2 were new variant strains of H5N 1 virus.The cleavage sites of HA from positive samples all possessed molecular characterization of highly pathogenic avian influenza virus.Mutation of key amino acids had been found among receptor binding sites,potential glycosylation sites,neutralizing epitopes and others.Conclusion It seemed evident that the H5N1 subtype viruses showed genetic diversifications and had undergone the evolution progress of multi-clade (2.3.2,2.3.4) to single caide (2.3.2) in the boundary regions of Yunnan province,during 2009 to July,2011.?
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