| 马江涛,陈慧,袁芳,马学旻,关光玉,詹军.宁夏地区1997-2011年急性弛缓性麻痹病例非脊髓灰质炎肠道病毒分型鉴定分析[J].中华流行病学杂志,2012,33(11):1163-1166 |
| 宁夏地区1997-2011年急性弛缓性麻痹病例非脊髓灰质炎肠道病毒分型鉴定分析 |
| Typing and identification of non-polio enterovirus from acute flaccid paralysis cases in Ningxia,1997-2011 |
| 收稿日期:2012-07-03 出版日期:2014-09-03 |
| DOI：10.3760/cma.j.issn.0254-6450.2012.11.015 |
| 中文关键词: 非脊髓灰质炎肠道病毒 分子生物学定型 流行病学 |
| 英文关键词: Non-polio enterovirus Molecular identification Epidemiology |
| 基金项目: |
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| 中文摘要: |
| 目的 对1997-2011年宁夏地区急性弛缓性麻痹(AFP)病例中分离的73株非脊髓灰质炎肠道病毒(NPEV)进行分型鉴定.方法 采用简并引物RT-PCR对分离株进行VP1区基因扩增和序列测定，测序结果进行BLAST比较和基因进化分析.结果 经过基因测序分析鉴定，73株NPEV中，有4株无法定型，另69株共包括27个血清型，分别属于A组肠道病毒(HEV-A)和HEV-B组，无HEV-C和HEV-D组毒株，其中HEV-A组毒株包括8个血清型23株，HEV-B组毒株包括19个血清型46株.69株病毒中，以EV71所占比例最大(9/69)，以CVA4、CVA16、Echo24、Echo6和CoxA9所占比例较高.结论 1997-2011年宁夏地区AFP病例分离的NPEV以HEV-B组为主要型别66.7％(46/69). |
| 英文摘要: |
| Objective To identify the serotype of 73 non-polio enterovirus (NPEV) strains from acute flaccid paralysis (AFP) cases in Ningxia province, during 1997-2011. Methods Partial sequencing of the VP1 region was amplified by RT-PCR with degenerate primers and sequenced while sequences were compared with the database of GenBank by the BLAST algorithm. Evolution was analyzed by constructing phylogenetic tree using Mega 5.1. Results In this study, a total of 73 NPEVs were analyzed, including 4 strains un-typed, 69 strains typed by RT-PCR. A total of 27 serotypes were identified, including 8 serotypes of human enterovirus (HEV)-A, 19 serotypes of HEV-B. The HEV-B group (46/69, 66.7％) constituted the largest proportion of isolates, followed by HEV-A (23/69, 33.3％), but no strains were found that belonged to HEV-C or HEV-D group. In the 69 strains, enterovirus 71 was the most frequently seen isolates, followed by coxsackie-virus A4, 16, 9 and echovirus 24, 6. Conclusion HEV-B was the most predominant (46/69, 66.7％) serotype of NPEV in Ningxia during the AFP surveillance, in 1997-2011. |
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