| 梁庆福,陈求扬,林淑芳,林建,逄宇,赵永,魏淑贞,王玉锋,郑金凤,赵雁林.福建省结核分枝杆菌多位点可变数目串联重复序列基因分型研究[J].中华流行病学杂志,2012,33(11):1167-1170 |
| 福建省结核分枝杆菌多位点可变数目串联重复序列基因分型研究 |
| Preliminary study on the MLVA genotyping of Mycobacterium tuberculosis isolates in Fujian province |
| 收稿日期:2012-05-25 出版日期:2014-09-03 |
| DOI：10.3760/cma.j.issn.0254-6450.2012.11.016 |
| 中文关键词: 结核分枝杆菌 多位点可变数目串联重复序列 基因分型 |
| 英文关键词: Mycobacterium tuberculosis Multiple loci VNTR analysis Genotyping |
| 基金项目:福建省自然科学基金(2010JOlll6)；福建省卫生厅青年科研课题(2009—2—36) |
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| 中文摘要: |
| 目的 了解福建省结核分枝杆菌的多位点可变数目串联重复序列基因分型(MLVA)的特征.方法 选择15个可变数目串联重复位点(VNTR)，检测福建省30个耐药监测点临床分离的结核菌株，结果使用BioNumerics (Version 4.5)软件进行聚类分析.结果 313株结核菌被分为9个基因群(Ⅰ～Ⅸ)，分别包含220、9、48、2、1、3、10、10、10株菌，以Ⅰ群为主(70.3％，220/313);Ⅰ群菌株异烟肼、链霉素、乙胺丁醇和耐多药的耐药率与其他基因群的差异无统计学意义(P＞0.05)，但利福平(RFP)耐药率为33.2％(73/220)，明显高于其他群菌株RFP的耐药率20.4％(19/93)，差异有统计学意义(P＜0.05).结论 福建省结核分枝杆菌菌株存在明显的基因多态性，以Ⅰ群菌株为主，并与RFP耐药性具有相关性，应加强此类菌株流行的监测. |
| 英文摘要: |
| Objective To preliminarily understand the genotyping characteristics regarding the variable number tandem repeats (VNTR) of Mycobacterium tuberculosis clinical isolates so as to provide evidence for the development of tuberculosis control and prevention programs in Fujian province. Methods Fifteen VNTR locus sets were used to detect the clinical isolates from the fifth surveillance project on tuberculosis resistance, in Fujian province. BioNumerics version 4.5 were used to analyze the cluster from the Results generated by genotyping. Results 313 Mycobacterium tuberculosis isolates were divided into 9 clusters, including Ⅰ, Ⅱ, Ⅲ, Ⅳ, Ⅴ, Ⅵ, Ⅶ, Ⅷ and Ⅸ, with the number of 220, 9, 48, 2, 1, 3, 10, 10, 10 isolates, respectively. Cluster Ⅰ was the major lineage, accounting for 70.3％ (220/313) of the total. Resistance rates of cluster Ⅰ isolates to isoniazid, streptomycin, ethambutol and multi-drug-resistaut were not statistically different from other clusters (P＞0.05). However, resistance rate to rifampicin (RFP) was significantly higher than that of other isolates of the clusters, 33.2％ (73/220) vs.20.4％ (19/93) (P＜0.05). Conclusion The strains isolated from Fujian province showed significant polymorphism on genotyping. Cluster Ⅰ seemed to be the dominant, calling for the close monitoring program on cluster Ⅰ strains. Results from our initial studies demonstrated the existence of significant correlation between cluster Ⅰ strains and drug resistance to RFP. |
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