文章摘要
张瑜,黄瑶,卜平.扬州地区腹泻型肠易激综合征与HTR3A、HTR3E基因多态性关联研究[J].中华流行病学杂志,2013,34(7):721-724
扬州地区腹泻型肠易激综合征与HTR3A、HTR3E基因多态性关联研究
Association between diarrhea.predominant irritable bowel syndrome and HTR3A, HTR3E gene polymorphism in Yangzhou.Jiangsu province, China
收稿日期:2013-01-22  出版日期:2014-09-17
DOI:10.3760/cma.j.issn.0254-6450.2013.07.014
中文关键词: 腹泻型肠易激综合征;基因;多态性;HTR3A;HTR3E
英文关键词: Diarrhea-predominant irritable bowel syndrome;Gene;Polymorphism;HTR3A;
基金项目:江苏省研究生创新工程项目(CXZZl2-0918);国家自然科学基金(8173392)
作者单位E-mail
张瑜 225009 扬州大学医学院 yizhangyu@yzu.edu.cn 
黄瑶 扬州市疾病预防控制中心  
卜平 225009 扬州大学医学院  
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中文摘要:
      目的探讨扬州地区门诊腹泻型肠易激综合征(D.IBS)与5-羟色胺受体3的亚型HTR3A、HTR3E基因非翻译区多态性的关系。方法采用聚合酶链反应-限制性片段长度多态性分析(PCR.RFLP)对450例D.IBS患者与300例健康对照者HTR3A基因5'端非翻译区C.-42C>T(rsl062613)和HTR3E基因3'非翻译区e.+76G>A(rs62625044)进行研究。结果男女性患者C.--42C>T多态性位点基因型分布较正常对照的差异有统计学意义(PA多态性位点G/A基因型和A等位基因的频率较正常对照均显著升高(P<0.05), 男性两组间的差异无统计学意义(P>0.05);与HTR3A基因的rsl062613位点CC基因型及HTR3E基因的rs62625044位点GG基因型相比, TT基因型及GA基因型与D-IBS密切相关, 风险系数分别为0.29(95%CI:0.14~0.61)、0.62(95%CI:0.39。0.99), 差异均有统计学意义。结论T等位基因和GA基因型可能分别是D.IBS患者和D.IBS女性患者的易感因素之-。
英文摘要:
      ObjectiveTo investigate the relationship between diarrhea-predominant irritable bowel syndrome(D-IBS)and HTR3A, HTR3E gene polymorphism in Yangzhou, Jiangsu province.MethodsPolymerase chain reaction(PCR)amplification and restriction fragment length pdymorphism(RFLP)technique were used to detect the 5'-UTR variant c.-42C>T of HTR3A and 3 7-UTR variant C.+76G>A of HTR3E on 300 healthy subjects and 450 patients with D-IBS.ResultsThere was significant difference noticed between the D-IBS patients and the controls in the gene type of C.-42C>T sites(P<0.05), while the frequency of T allele was significantly higher in both female and male patients than that in the controls(P<0.05).The frequencies of G/A gene type and A allele of C.+76G>A sites were significantly higher in the female-patient group than that in the controls(P<0.05), however, there was no significant difference between those male patients and controls(P>0.05).When comparing to the CC gene type of rsl062613 site and GG gene type of rs62625044 site.the TT and GA gene type were closely related to the D-IBS, with the risk coefficients as 0.29(95%CI:0.14-0.61)and 0.62(95%C/:0.39-0.99), with statistically significant differences.ConclusionT allele and GA gene type might respectively serve as the predisposing factors of D-IBS and on the female D-IBS patients.
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