| 王继宝,张娜,于海英,李艳玲,段星,颜浩,杨锦,段松,蒋岩.免疫印迹法带型作为判定HIV-1 新发感染的研究[J].中华流行病学杂志,2013,34(10):998-1002 |
| 免疫印迹法带型作为判定HIV-1 新发感染的研究 |
| Study on the role of western blot band profile for the detection of recent HIV infection |
| 收稿日期:2013-05-21 出版日期:2014-10-08 |
| DOI：10．3760/cma．j．issn．0254—6450．2013．10．013 |
| 中文关键词: HIV新发感染|免疫印迹法带型 |
| 英文关键词: Recent HIV infection| Westem blot band |
| 基金项目:国家科技重大专项(2012zxl0001001—001—002) |
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| 中文摘要: |
| 目的从HIV确证的免疫印迹法(wB)带型中获得新发感染相关信息。方法收 集云南省德宏州2011年经WB确证为HIV抗体阳性样本1466份，分别用BED捕获酶免疫法 (BED．CEIA)和限制性抗原亲和力酶联法(LAg．Avidity EIA)检测新发感染者，分析比较WB检测 中不同条带特征对新发感染判定的意义。结果BED．CEIA或LAg．Avidity EIA检出的新发感染 者，其WBI拘p17、p24、p31、gp41、p51、p55、p66、gpl20和Igpl60条带反应强度与长期感染的差异有 统计学意义(P<0．05)，均低于长期感染者相应的条带反应强度。结论WB条带中的p31呈阴性 反应、WB条带反应强度总分不高于13分、eny区条带反应强度不高于5分或pol区条带反应强度 不高于4分时应高度怀疑其为新发感染。 |
| 英文摘要: |
| Objective To obtain information on HIV disease progression through western blot(WB)band profile．Methads 1466 available serum samples of newly reported HIV，AIDS cases，confirmed by WB from Dehong prefecture of Yunnan province in 20 1 l，were tested，using the BED HIV Incidence Capture Enzyme Immunoassay(BED．CEIA)and Limiting Antigen Avidity Enzyme Immunoassay(LAg·Avidity EIA)，respectively．Differences of WB band profiled between recent infections and established infections were then compared．Results Infections defined as ‘recent’showed lower mean antibody intensities to p17，p24，p31，gp41，p51，p55，p66，gpl20 and gpl60 in WB when compared to those established infections with statistical difference(P<0．05)．ConclusionThese WB band profiles which were considered as‘recent’infections would include factors as：lack of antibody reactivity to gp41 antigen，with intensity scores of all bands lower than 1 3， and those intensity scores of antibody to env protein lower than 5 or to pol protein lower than 4． |
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