| 杨卉娟,刘建生,张云昆,马忠飞,赵卫中,潘明,陈俊英,邵聪文,马绍辉.2009年云南省柯萨奇病毒B3型分离株A103/KM/09全基因序列分析[J].中华流行病学杂志,2013,34(12):1208-1212 |
| 2009年云南省柯萨奇病毒B3型分离株A103/KM/09全基因序列分析 |
| Complete nueleotide sequence of a human coxsaekievirus B3 strain A103/KM/09 isolated inYunnan province,2009 |
| 收稿日期:2013-08-01 出版日期:2014-10-16 |
| DOI：10.3760/cmaj.issn.0254-6450.2013.012.012 |
| 中文关键词: 柯萨奇病毒B3型 全基因序列 序列分析 |
| 英文关键词: Coxsackievirus B3 Complete genome Sequence analysis |
| 基金项目:云南省应用基础研究项目(2013FZl36) |
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| 中文摘要: |
| 目的 分析2009年云南省脑炎患儿粪便标本分离的柯萨奇病毒B3型分离株A103/KM/09全基因序列,了解其遗传特性。方法 设计针对柯萨奇病毒B3型引物,提取病毒RNA、RT-PCR扩增和产物直接测序获得全基因组序列,利用Geneious和Mega 5.05软件进行核苷酸、氨基酸序列比对及其系统进化分析,应用RDP 3和SimPlot 3.5.1重组软件对序列进行重组分析。结果 该分离株全基因组核苷酸序列长度为7389 bP。其核苷酸和氨基酸序列与其原型株Nancy的同源性分别为81.O%和95.7%；与其他柯萨奇病毒B3型毒株的各区段核苷酸和氨基酸同源性分别为81.0%~88.0%和95.7%~98.0%；进化分析发现CVB3可分为5个分支(I~V),核苷酸之间的差异为16.2%~24.3%；中国分离株属V分支,又可分为A～D亚分支,核苷酸之间的差异为4.3%~11.4%。并发现A103/KM/09株基因序列在非结构区可能存在重组事件。结论柯萨奇病毒B3型分为5个基因型,云南分离株A103/KM/09属于V-D亚型,而中国分离株已经发生一定进化。 |
| 英文摘要: |
| Objective To analyze the genetic characterization ofthe complete genome from ahuman coxsackievirus B3 strain A103KM/09 isolated in Yunnan province，2009.Methods Byusing RT-PCR，all the eight fragments which containing about 1 000 nucleotides and covering full viralgenome，were sequenced.By using Mega 5.05，Geneious，RDP 3 and SimPlot 3.5.1 software，sequences were aligned with other enterovirus reference sequences.Phylogenetic and recombinationanalysis were also carried out.Results The A103KM的9 isolate genome showed 7389 nucleotides inlength，encoding for 2 1 85 amino acids.In the complete genome，the homology of nucleotideand amino acid among the seven coxsackievirus B3 isolates were 81.O%-88.0%and 95.7%～98.0%.respectively.There appeared 81.0%and 95.7%homology when compared with that of Nancyprototype strain.Results from the Phylogenetic analysis showed that the coxsackievirus B3 formedfive distinct clades.I-V.Nucleotide divergence rates between elades were 1 6.2%-24.3%.TheA103/KM/09 strain belonged to clade V.Clade V was further divided into four sub.clades.A-D.The nucleotide divergence between sub-clades was 4.3%-11.4%.Putative recombinant event forAl03/KM09 was detected.Conclusion Aneoxsackievirus B3 isolates could be divided into five clades，with A103/KM09 strain belonged to C1ade V-D.Evolution of coxsackievirus B3 had occurred inChina. |
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