| 张越伦,张欢,詹思延.如何撰写高质量的流行病学研究论文第五讲医院感染暴发报告和干预研究的透明报告规范——ORION介绍[J].中华流行病学杂志,2014,35(2):208-211 |
| 如何撰写高质量的流行病学研究论文第五讲医院感染暴发报告和干预研究的透明报告规范——ORION介绍 |
| How to write high-quality epidemiological research paper V . Guidelines for Transparent Reporting of Outbreak Reports and Intervention Studies of Nosocomial Infection (ORION statement ) |
| 收稿日期:2013-12-20 出版日期:2014-09-17 |
| DOI：10.3760/cma.j.issn.0254-6450.2014.02.024 |
| 中文关键词: 医院感染暴发报告和干预研究的透明报告规范 医院感染 清单 |
| 英文关键词: ORION statement Nosocomial infection List |
| 基金项目: |
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| 中文摘要: |
| 医院感染已成为增加医疗负担的重要原因。随着医院感染问题日趋严重，越来越多的研究者开展相关领域的研究，以期为医院感染防控工作提供参考和建议。然而相关研究的报告质量参差不齐，限制了这些研究结果的理解和进一步利用。本文介绍“医院感染暴发报告和干预研究的透明报告规范”（Guidelines for Transparent Reporting ofOutbreak Reports and InterventionStudies ofNosocomial Infection，ORION statement），并解读报告规范中一些重要的条目。 |
| 英文摘要: |
| Objective To develop a real-time PCR assay for the rapid identification of Clostridium（C.） difficile and its toxin. Methods TaqMan real-time PCR was developed for the rapid identification of species specific gene（tpi）of C. difficile strains and the toxins A（TcdA），B（TcdB） and truncated toxin A（TcdAT）. Sensitivity，specificity and anti-interference ability of these methods were estimated，as well. Feces sampled from fifty diarrhea patients were tested by real-time PCR and compared to the results from VIDAS assay. Results The detection limits of tpi were 6×10-2 CFU/μland 6 × 10-1CFU/μ l in the non-oxin producing and toxin producing strains，respectively. The coefficients of variability（CV）of intra-assay and inter-assay for the detection limits of tpi in the non-toxin producing strain were 2.1% and 2.3% . The CVs of intra-assay and inter-assay for the detection limit of tpi，tcdA，tcdB and tcdAT in the toxin producing strain were 3.0% and 3.4%，2.9% and 3.2%，5.3% and 5.7%，2.7% and 2.8%，respectively. No interferance was detected from other genus or species in clostridium. From 50 clinical samples，thirty-nine of them were negative and six of them were positive under the TaqMan-MGB probe technique in accordance with VIDAS. Five samples appeared positive using the TaqMan-MGB probe technique，in which 3 were dubious and 2 were negative under VIDAS. Conclusion The newly developed method was a sensitive and reliable assay for rapid identification of C. difficile and its toxin. This method could be used to screen C. difficile isolates harboring truncated toxin A to avoid misdiagnosis，clinically. |
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