| 张磷,王远志,陈创夫,李永祥,张科,杜景云,张亚丽,车召堂.新疆北疆部分地区硬蜱伯氏疏螺旋体基因型研究[J].中华流行病学杂志,2014,35(3):262-265 |
| 新疆北疆部分地区硬蜱伯氏疏螺旋体基因型研究 |
| Isolation of Borrelia burgdorferi in ixodes from four counties，in North Xinjiang |
| 收稿日期:2013-09-09 出版日期:2014-09-18 |
| DOI： |
| 中文关键词: 伯氏疏螺旋体 基因型 硬蜱 |
| 英文关键词: Borrelia burgdorferi Sensu Stricto Genotype Ixodidae |
| 基金项目:国际科技合作项目(2013DFA32380)；国家自然科学基金(31060334)；国家科技支撑计划(2013BAl05805) |
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| 中文摘要: |
| 目的调查和鉴定新疆北疆石河子市、沙湾县、伊宁县和察布查尔县媒介蜱，并确定其携带的莱姆病螺旋体的基因型。方法选择4县市6个牧区为调查点，采集羊源寄生硬蜱，结合形态学与16S rRNA进行蜱种鉴定；采用BSK-H培养基对莱姆病螺旋体分离培养，并用硝酸银染色和巢式PCR法进行病原检测，阳性产物测序结果比对，并与11种GenBank参考序列比对，确定莱姆病螺旋体的基因型。结果6个调查点共采集寄生硬蜱900多只，经形态学与16S rRNA鉴定分别为图兰扇头蜱、刻点血蜱、亚洲璃眼蜱和边缘革蜱；从中分别选取样本硬蜱共102只，分24管，分离培养后结合巢式PCR和硝酸银染色共获得16管阳性培养物。5S～23S rRNA基因问隔区测序比对分析表明，所有菌株为伯氏疏螺旋体与国际报道的B.burgdoq'eri Sensu Stricto(B31)基因型具有高度同源性，同源性为98.6%～99.5%；OspC基因型分析与上述结果一致。结论4县市分离获得伯氏疏螺旋体，基因型为B.burgdooCeri Sensu Stricto，且存在生物多样性。从图兰扇头蜱中分离到伯氏疏螺旋体为我国首次报道。 |
| 英文摘要: |
| Objective To identify ticks and determine the Bormlia(B.)burgd09reri genotype from four counties of northern Xinjiang.Methods SheeD ticks were collectedom 6 surveillance sites in four counties including Shihezi.Shawan.Yining and Chabuchaer.All ticks were initiallyscreened out based on morphological Methods and l 6S rRNA sequence analysis.B.burgdori wasdetected and cultivated with BSK-H medium.Combined with nested PCR，silver nitrate staining was employed to detect B.burgdorferi.Genotype of isolated B.burgdo咖-was determined bySequencing and phylogenic analysis based on 1 l conference sequences.Results Hyalommaasiaticum asiaticum，Haemaphysalis punctata，Dermaeentor manatus and Rhipieephalus turanieuswere identified from more than 900 ticks.Out of 24 tubes from 102 representative tick specimens.1 6 tube were positive for B.burgdo咖r.Sequencing of 5S-23S rRNA intergenic spacer showed98.6%-99.5%identities to B.burgdo以ri Sensu Stficto(B3 1).Results from the analysis of OspCgenotype showed consistent with that of 5S-23S rRNA intergenic spacer.Conclusions 16 strains ofB.burgdo啦ri Sensu Stricto were isolated in four counties，from northern Xinjiang.Additionally， B.burgdorferi Sensu Stricto was isolated from Rhipicephalus turanicus first time in China. |
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