文章摘要
张磷,王远志,陈创夫,李永祥,张科,杜景云,张亚丽,车召堂.新疆北疆部分地区硬蜱伯氏疏螺旋体基因型研究[J].中华流行病学杂志,2014,35(3):262-265
新疆北疆部分地区硬蜱伯氏疏螺旋体基因型研究
Isolation of Borrelia burgdorferi in ixodes from four counties,in North Xinjiang
收稿日期:2013-09-09  出版日期:2014-09-18
DOI:
中文关键词: 伯氏疏螺旋体;基因型;硬蜱
英文关键词: Borrelia burgdorferi Sensu Stricto;Genotype;Ixodidae
基金项目:国际科技合作项目(2013DFA32380);国家自然科学基金(31060334);国家科技支撑计划(2013BAl05805)
作者单位E-mail
张磷 医学院病原生物学与免疫学教研室, 石河子大学 832000 wyzshz@sina.com ccf-xb@163.com 
王远志 医学院病原生物学与免疫学教研室, 石河子大学 832000  
陈创夫 动物科技学院人兽共患病研究室  
李永祥 医学院病原生物学与免疫学教研室, 石河子大学 832000  
张科 动物科技学院人兽共患病研究室  
杜景云 医学院病原生物学与免疫学教研室, 石河子大学 832000  
张亚丽 医学院病原生物学与免疫学教研室, 石河子大学 832000  
车召堂 动物科技学院人兽共患病研究室  
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中文摘要:
      目的调查和鉴定新疆北疆石河子市、沙湾县、伊宁县和察布查尔县媒介蜱,并确定其携带的莱姆病螺旋体的基因型。方法选择4县市6个牧区为调查点,采集羊源寄生硬蜱,结合形态学与16S rRNA进行蜱种鉴定;采用BSK-H培养基对莱姆病螺旋体分离培养,并用硝酸银染色和巢式PCR法进行病原检测,阳性产物测序结果比对,并与11种GenBank参考序列比对,确定莱姆病螺旋体的基因型。结果6个调查点共采集寄生硬蜱900多只,经形态学与16S rRNA鉴定分别为图兰扇头蜱、刻点血蜱、亚洲璃眼蜱和边缘革蜱;从中分别选取样本硬蜱共102只,分24管,分离培养后结合巢式PCR和硝酸银染色共获得16管阳性培养物。5S~23S rRNA基因问隔区测序比对分析表明,所有菌株为伯氏疏螺旋体与国际报道的B.burgdoq'eri Sensu Stricto(B31)基因型具有高度同源性,同源性为98.6%~99.5%;OspC基因型分析与上述结果一致。结论4县市分离获得伯氏疏螺旋体,基因型为B.burgdooCeri Sensu Stricto,且存在生物多样性。从图兰扇头蜱中分离到伯氏疏螺旋体为我国首次报道。
英文摘要:
      Objective To identify ticks and determine the Bormlia(B.)burgd09reri genotype from four counties of northern Xinjiang.Methods SheeD ticks were collectedom 6 surveillance sites in four counties including Shihezi.Shawan.Yining and Chabuchaer.All ticks were initiallyscreened out based on morphological Methods and l 6S rRNA sequence analysis.B.burgdori wasdetected and cultivated with BSK-H medium.Combined with nested PCR,silver nitrate staining was employed to detect B.burgdorferi.Genotype of isolated B.burgdo咖-was determined bySequencing and phylogenic analysis based on 1 l conference sequences.Results Hyalommaasiaticum asiaticum,Haemaphysalis punctata,Dermaeentor manatus and Rhipieephalus turanieuswere identified from more than 900 ticks.Out of 24 tubes from 102 representative tick specimens.1 6 tube were positive for B.burgdo咖r.Sequencing of 5S-23S rRNA intergenic spacer showed98.6%-99.5%identities to B.burgdo以ri Sensu Stficto(B3 1).Results from the analysis of OspCgenotype showed consistent with that of 5S-23S rRNA intergenic spacer.Conclusions 16 strains ofB.burgdo啦ri Sensu Stricto were isolated in four counties,from northern Xinjiang.Additionally, B.burgdorferi Sensu Stricto was isolated from Rhipicephalus turanicus first time in China.
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