HLA-DQ基因多态性与HBV感染不同临床结局的关联研究

刘文宣; 张晓琳; 高霞; 杨磊; 李保欣; 刘殿武

文章摘要

刘文宣,张晓琳,高霞,杨磊,李保欣,刘殿武.HLA-DQ基因多态性与HBV感染不同临床结局的关联研究[J].中华流行病学杂志,2016,37(3):384-388

HLA-DQ基因多态性与HBV感染不同临床结局的关联研究

Association between HLA-DQ gene polymorphisms and different outcomes of hepatitis B virus infection

收稿日期:2015-11-03 出版日期:2016-03-15

DOI：10.3760/cma.j.issn.0254-6450.2016.03.019

中文关键词: 肝炎病毒,乙型人类白细胞抗原单核苷酸多态性

英文关键词: Hepatitis B virus Human leucocyte antigen Single nucleotide polymorphism

基金项目:河北省科技计划项目(132777246;13277732D)

作者	单位	E-mail
刘文宣	050017 石家庄, 河北医科大学流行病与卫生统计学教研室
张晓琳	050017 石家庄, 河北医科大学流行病与卫生统计学教研室
高霞	050017 石家庄, 河北医科大学流行病与卫生统计学教研室
杨磊	050017 石家庄, 河北医科大学流行病与卫生统计学教研室
李保欣	050031 石家庄, 河北医科大学第一附属医院传染病科
刘殿武	050017 石家庄, 河北医科大学流行病与卫生统计学教研室	liudw56@tom.com

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中文摘要:

目的研究人类白细胞抗原HLA-DQ基因rs2856718和rs9275572两个位点突变是否与HBV感染的不同结局有关。方法采用问卷调查收集825例研究对象的一般资料,同时收集其外周血,提取DNA,用基质辅助激光解吸电离飞行时间质谱实验平台进行DNA的单核苷酸多态性分型,使用单因素及多因素logistic回归分析方法分析HLA-DQ基因多态性与HBV感染不同结局的关系。结果携带HLA-DQ基因rs2856718G(524例)和rs9275572A(369例)的个体感染HBV的风险降低(OR=0.702,95%CI:0.558~0.856,P=0.001;OR=0.548,95%CI:0.365~0.847,P=0.005),且HBV自然清除能力强(OR=0.589,95%CI:0.478~0.892,P=8.81×10^-3;OR=0.673,P=0.014,95%CI:0.457~0.860)。携带rs9275572A的个体由慢性乙型肝炎和肝硬化进展到原发性肝细胞癌的风险降低(OR=0.759,95%CI:0.538~0.914,P=0.041)。结论在中国北方汉族人群中,HLA-DQ的两个SNP位点(rs9275572和rs2856718)与HBV感染后的不同结局有关,rs9275572A和rs2856718G为HBV感染的保护性因素,在HBV自然清除和原发性肝细胞癌的进展中起到保护作用。

英文摘要:

Objective To understand the association between two single-nucleotide polymorphism (HLA-DQ rs28567185 and rs9275572) and different outcomes of hepatitis B virus (HBV) infection. Methods A total of 825 HBV infection related cases were enrolled, and peripheral blood samples were collected from them for DNA extraction. The single-nucleotide polymorphism in HLA-DQ region were genotyped by using matrix-assisted laser desorption/ionization time of flight mass spectrometry. Logistic regression analysis was conducted to evaluate the association between HLA-DQ gene polymorphism and different outcomes of hepatitis B virus infection. Results Our study indicated that cases with HLA-DQ rs2856718G (524 cases) and rs9275572A (369 cases) had reduced susceptibility to HBV infection (OR=0.702, 95%CI: 0.558-0.856, P=0.001; OR=0.548, 95%CI:0.365-0.847, P=0.005) and higher HBV natural clearance (OR=0.589, 95%CI: 0.478-0.892, P=8.81×10-3; OR=0.673, 95%CI: 0.457-0.860, P=0.014). Moreover, rs9275572A was also associated with the lower risk of the development of hepatocellular carcinoma (OR=0.759, 95%CI: 0.538-0.914, P=0.041). Conclusion Our study suggested that HLA-DQ loci might be associated with the different outcomes of HBV infection in Chinese in Han ethnic group in northern China, rs2856718G and rs9275572A might be protective factors for HBV infection, HBV natural clearance and HBV- related disease progress.

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