文章摘要
王闻卿,王多春,朱林英,傅益飞,郝莉鹏,许学斌,苏靖华,傅慧琴,叶楚楚,孙乔,郑英杰.上海市浦东新区腹泻患者气单胞菌流行特征及毒力基因研究[J].中华流行病学杂志,2016,37(3):402-405
上海市浦东新区腹泻患者气单胞菌流行特征及毒力基因研究
Infection status and virulent genes of Aeromonas in diarrhea patients in Pudong New Area, Shanghai
收稿日期:2015-08-05  出版日期:2016-03-15
DOI:10.3760/cma.j.issn.0254-6450.2016.03.023
中文关键词: 气单胞菌;腹泻;混合感染;毒力基因
英文关键词: Aeromonas;Diarrhea;Co-infection;Virulent gene
基金项目:
作者单位E-mail
王闻卿 200136 上海市浦东新区疾病预防控制中心微生物检验科  
王多春 102206 北京, 中国疾病预防控制中心传染病预防控制所, 传染病预防控制国家重点实验室  
朱林英 200136 上海市浦东新区疾病预防控制中心微生物检验科  
傅益飞 200136 上海市浦东新区疾病预防控制中心传染病防治与消毒管理科  
郝莉鹏 200136 上海市浦东新区疾病预防控制中心微生物检验科  
许学斌 200336 上海市疾病预防控制中心微生物检验科  
苏靖华 200136 上海市浦东新区疾病预防控制中心微生物检验科  
傅慧琴 200136 上海市浦东新区疾病预防控制中心微生物检验科  
叶楚楚 200136 上海市浦东新区疾病预防控制中心传染病防治与消毒管理科  
孙乔 200136 上海市浦东新区疾病预防控制中心传染病防治与消毒管理科 qsun@pdcdc.sh.cn 
郑英杰 200032 上海, 复旦大学公共卫生学院教育部公共卫生重点实验室, 复旦大学公共卫生学院卫生微生物学教研室 yjzheng@shmu.edu.cn 
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中文摘要:
      目的 了解上海市浦东新区腹泻患者气单胞菌流行特征及毒力基因特征。方法 2012年1-12月,在上海市浦东新区12家腹泻监测点医院对符合病例定义的病例采集粪便,进行13种腹泻病原体检测,对分离到的气单胞菌株进行5种与腹泻相关的毒力基因(ast、aerA、act、alt、hlyA)检测。结果 2 533例腹泻患者中,101例感染气单胞菌,阳性率为4.0%,分离到嗜水气单胞菌17株(18.8%),维隆气单胞菌温和变种44株(52.5%),豚鼠气单胞菌12株(29.7%)。44例患者为气单胞菌与其他病原体混合感染。夏季为高发季节,≥20岁人群高发。71例(70.3%)患者临床症状为水样便,20例(19.8%)患者出现呕吐症状,11例(10.9%)有发热症状。95.0%的气单胞菌携带毒力基因,hlyA基因阳性率为5.9%,aerA基因阳性率为6.9%,act基因阳性率为67.3%,alt基因阳性率为42.6%,ast基因阳性率为13.9%。结论 浦东新区腹泻患者中气单胞菌感染阳性率较高,并存在较高比例的混合感染。绝大多数气单胞菌都携带毒力基因,且分布存在差异性。
英文摘要:
      Objective To investigate the infection status and virulent genes of Aeromonas in patients with acute diarrhea in Pudong New Area, Shanghai. Methods In 2012, stool samples were collected from diarrhea patients in 12 sentinel hospitals in Pudong for the detections of 13 pathogens causing diarrhea, and the detections of 5 diarrhea related virulent genes were conducted for Aeromonas isolates. Results A total of 101 patients were infected with Aeromonas in 2 533 patients (4.0%). A total of 101 Aeromonas strains were isolated, including 17 Aeromonas hydrophila strains (18.8%), 44 Aeromonas veronii biovar sobria strains (52.5%) and 12 Aeromonas caviae strains (29.7%). And 44 co-infections with other pathogens were detected. Aeromonas infection mainly occurred in summer and in people aged ≥20 years. Among the patients infected with Aeromonas, 71(70.3%) had watery diarrhea, 20(19.8%) had vomiting and 11(10.9%) had fever. Virulent genes detection showed that 95.0% of the Aeromonas. strains carried virulent genes, and the detection rates of hlyA, aerA, act, alt, and ast genes were 5.9%, 6.9%, 67.3%, 42.6% and 13.9%, respectively. Conclusions High incidence of Aeromonas infection was found in the patients with acute diarrhea in Pudong, and a high proportion of co-infections with other pathogens was detected too. Most Aeromonas strains carried virulent genes, and the distribution varied.
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