文章摘要
李鲲,林献丹,李明慧,王淼若,孙肖瑜,张永振.浙江省啮齿动物中温州病毒的基因组学分析[J].中华流行病学杂志,2017,38(3):384-387
浙江省啮齿动物中温州病毒的基因组学分析
Genomic analysis of Wenzhou virus in rodents from Zhejiang province
收稿日期:2016-08-24  出版日期:2017-03-18
DOI:10.3760/cma.j.issn.0254-6450.2017.03.022
中文关键词: 温州病毒;啮齿动物;新亚型;呼吸道疾病
英文关键词: Wenzhou virus;Rodent;Novel variant;Respiratory disease
基金项目:国家自然科学基金(81290343,81273014)
作者单位E-mail
李鲲 102206 北京, 中国疾病预防控制中心传染病预防控制所人兽共患病室  
林献丹 325000 浙江省温州市疾病预防控制中心消毒和病媒生物防制所  
李明慧 102206 北京, 中国疾病预防控制中心传染病预防控制所人兽共患病室  
王淼若 323700 龙泉市疾病预防控制中心业务管理科  
孙肖瑜 325000 浙江省温州市疾病预防控制中心消毒和病媒生物防制所  
张永振 102206 北京, 中国疾病预防控制中心传染病预防控制所人兽共患病室 zhangyongzhen@icdc.cn 
摘要点击次数: 2248
全文下载次数: 831
中文摘要:
      目的 为了解国内发现的新型沙粒病毒(温州病毒)在温州地区啮齿类动物中的流行情况。方法 采用巢式RT-PCR方法分别对采集自温州(48份)及龙泉(156份)的啮齿动物样本筛查沙粒病毒。结果 在温州地区采集的5份啮齿动物样本中再次检测到温州病毒,阳性率为10.41%;但在龙泉地区采集的156份样本中均未发现温州病毒。分析病毒的全基因组序列发现其中4株病毒与已知的温州病毒有很高的同源性;而编号为Wencheng-Rn-288病毒株的S和L节段与已知病毒同源性分别为87.5%和91.6%,在进化树上为一独立分支,为温州病毒的新亚型。结论 温州病毒在温州地区啮齿动物中有较高的流行率,且呈多样性。鉴于在柬埔寨已发现温州病毒能引起人呼吸道疾病,因此我国应加强温州病毒的监测。
英文摘要:
      Objective Arenavirus is a negative single-stranded RNA virus and an important human pathogen, mainly harbored and transmitted by rodents, causing severe diseases, including hemorrhagic fever and encephalitis. Following the discovery of a novel pathogenic arenavirus (Wenzhou virus, WENV), the prevalence of WENV in local small rodents was investigated. Methods By using RT-PCR, WENV was screened in 48 and 156 rodents sampled from Wenzhou and Longquan, respectively. Results Consequently, WENV was detected in 5 (10.41%) rodents sampled from Wenzhou. However, no WENV was identified in all the rodents sampled from Longquan. Genetic analysis of complete genome sequences indicated that 4 of 5 virus strains were closely related to the known Wenzhou viruses with high homology. Especially, the L and S segments of Wencheng-Rn-288 strain shared homology of 87.5% and 91.6% with other viruses, respectively. They formed a distinct lineage, suggesting that this strain might be a novel variant of WENV. Conclusions Our results indicate that WENV has a high prevalence and high genetic diversity among rodents in Wenzhou. As the respiratory disease caused by WENV has been detected in Cambodia, it is necessary to strengthen the surveillance for WENV in China.
查看全文   Html全文     查看/发表评论  下载PDF阅读器
关闭