| 石丽媛,丁奕博,谭红丽,郭英,张海鹏,段存娟,李伟,王鹏.云南省鹤庆县2017年分离鼠疫菌分子溯源[J].中华流行病学杂志,2018,39(7):983-987 |
| 云南省鹤庆县2017年分离鼠疫菌分子溯源 |
| Source tracing of the Yersinia pestis strains isolated from Heqing county, Yunnan province in 2017 |
| 收稿日期:2018-01-31 出版日期:2018-07-17 |
| DOI：10.3760/cma.j.issn.0254-6450.2018.07.022 |
| 中文关键词: 鼠疫 鹤庆县 多位点可变数目串联重复序列分析 |
| 英文关键词: Plague Heqing Multiple-locus variable-number tandem repeat analysis |
| 基金项目:国家自然科学基金（81160354）；云南省中青年学术和技术带头人后备人才培养项目（2014HB037）；云南省医学学科带头人培养项目（D-201652） |
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| 中文摘要: |
| 目的 了解2017年云南省鹤庆县新分离的鼠疫菌的基因分型，为该地的鼠疫防控提供科学依据。方法 采用差异片段（DFR）、规律成簇的间隔短回文重复序列（CRISPRs）和多位点可变数目串联重复序列分析（MLVA）3种方法对10株鹤庆县新分离鼠疫菌进行分型，并将10株鼠疫菌及邻近疫源地鼠疫菌株93株纳入聚类分析。结果 鹤庆鼠疫菌株与丽江鼠疫疫源地菌株具有相同的DFR型（Genomovar 05型）及CRISPRs型（Ca7簇，22型），在MLVA聚类分析中，鹤庆鼠疫菌株与丽江野鼠鼠疫菌株位于同一个簇，两者之间仅有2个位点（N2117，M23）的差异。结论 2017年云南省鹤庆鼠疫菌株与丽江野鼠鼠疫疫源地菌株具有很高的同源性，鹤庆县疫情可能是丽江鼠疫进一步向南扩散的结果。 |
| 英文摘要: |
| Objective To understand the genotype of the Yersinia (Y.) pestis strains isolated from Heqing county, Yunnan province in 2017 and provide evidence for the prevention and control of plague in this area. Methods Ten Y. pestis strains isolated from Heqing were typed by the detections of different region (DFR) and clustered regularly interspaced short palindromic repeats (CRISPRs) as well as multiple-locus variable-number tandem repeat analysis (MLVA). And the results were compared with those of the 93 Y. pestis strains from the adjacent plague foci of Heqing obtained from the established database for clustering analysis. Results The results showed that Heqing strains had the same type of DFR (Genomovar 05) and CRISPRs (Cluster Ca7, Type 22) with isolates from the plague focus in Lijiang. Heqing strains and Lijiang strains were in the same cluster in MST and only VNTR loci N2117 and M23 of Heqing strains were different from that of Lijiang strains. Conclusion The Y. pestis strains isolated from Heqing in 2017 were highly homogenous with the strains isolated from wild rodents in plague focus in Lijiang, and Heqing plague might be the result of further southward spread of Lijiang plague. |
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