文章摘要
刘佳,耿杰,刘家琪,薛秀娟,闫江舟,袁源,张向兵,刘春华,张国龙.河南省部分地区HIV/AIDS肠道微生物群落结构的相关因素分析[J].中华流行病学杂志,2022,43(4):566-571
河南省部分地区HIV/AIDS肠道微生物群落结构的相关因素分析
Analysis of factors associated with the structure of the gut microbial community in HIV/AIDS patients in some areas of Henan province
收稿日期:2021-10-25  出版日期:2022-04-16
DOI:10.3760/cma.j.cn112338-20211025-00816
中文关键词: 艾滋病|艾滋病病毒|肠道微生物|相关因素
英文关键词: AIDS|HIV|Gut microbes|Related factors
基金项目:国家科技重大专项(2018ZX0715-009);河南省科技攻关计划(182102310236)
作者单位E-mail
刘佳 河南省疾病预防控制中心性病艾滋病防治研究所, 郑州 450016  
耿杰 河南省疾病预防控制中心性病艾滋病防治研究所, 郑州 450016  
刘家琪 河南省疾病预防控制中心性病艾滋病防治研究所, 郑州 450016  
薛秀娟 河南省疾病预防控制中心性病艾滋病防治研究所, 郑州 450016  
闫江舟 河南省疾病预防控制中心性病艾滋病防治研究所, 郑州 450016  
袁源 河南省疾病预防控制中心性病艾滋病防治研究所, 郑州 450016  
张向兵 河南省疾病预防控制中心性病艾滋病防治研究所, 郑州 450016  
刘春华 河南省疾病预防控制中心性病艾滋病防治研究所, 郑州 450016  
张国龙 河南省疾病预防控制中心性病艾滋病防治研究所, 郑州 450016 1296190445@qq.com 
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中文摘要:
      目的 分析河南省HIV感染者/AIDS病例(HIV/AIDS)肠道微生物群落结构的相关因素。方法 在河南省采取方便抽样方法抽取122例抗病毒治疗和未抗病毒治疗的病例,采集全血和粪便标本。提取粪便样本的基因组DNA,采用Illumina NovaSeq 6000高通量测序系统对16S rRNA的V3~V4高变区域进行测序。主要在菌群的属水平进行分析,选取丰度最高的30个属作为肠道微生物群落结构的衡量指标。使用冗余分析和Envfit函数对群落结构和各因素之间的相关性进行分析。结果 本研究完成了122例病例的16S rRNA测序和分析,其基本情况为:平均BMI为(23.62±2.78)kg/m2,年龄(47±13)岁,男性占66.39%(81/122),以异性性传播为主要感染途径(51.64%,63/122),有36例(29.51%,36/122)病例未接受抗病毒治疗。在122例总人群病例样本中,前5位优势菌属是Prevotella、Roseburia、Megamonas、BacteroidesFaecalibacterium。在86例接受抗病毒治疗的病例样本中,前5位优势菌属是Prevotella、Megamonas、Bacteroides、RoseburiaFaecalibacterium。在36例未抗病毒治疗病例样本中,前5位优势菌属是Prevotella、Faecalibacterium、Roseburia、BacteroidesMegamonas。在总人群中,抗病毒治疗(P<0.001)是群落结构最显著的相关因素。其他显著因素有:确诊时间(P=0.009)、病毒载量(P=0.022)和HCV抗体(P=0.018)。抗病毒治疗与Megamonas呈正相关,与Prevotella、RoseburiaFaecalibacterium呈负相关,而确诊时间、病毒载量和HCV抗体3个因素则与Prevotella、RoseburiaFaecalibacterium呈正相关,与Megamonas呈负相关。在未抗病毒治疗人群中,确诊时间(P=0.003)是与群落结构显著相关的因素。确诊时间与Roseburia、Faecalibacterium、MegamonasPrevotella呈正相关,与Bacteroides呈负相关。结论 抗病毒治疗和确诊时间是与肠道微生物群落结构显著相关的因素,并且对多个高丰度菌属有着重要影响。
英文摘要:
      Objective To investigate the related factors associated with the structure of the gut microbial community in HIV infection/AIDS cases (HIV/AIDS) in Henan province. Methods The convenience sampling method was used to select 122 cases who were receiving Antiviral Treatment (ART) or ART-naive in Henan. Whole blood and stool specimens were collected. Genomic DNA of stool samples was extracted, and the V3-V4 hypervariable regions of the 16S rRNA gene were sequenced using Illumina NovaSeq 6000 high-throughput sequencing system. The analysis was performed mainly at the genus level, and the 30 genera with the highest abundance were selected as a measure of the gut microbial community structure. The correlation between community structure and related factors was analyzed using redundancy analysis and Envfit function. Results 122 cases were finally completed sequencing and analysis, the average BMI was (23.62±2.78) kg/m2 and the average age was (47±13) years. Among them, male accounted for 66.39% (81/122), and heterosexual transmission route constituted the largest ratio, accounting for 51.64% (63/122). 36 cases were treatment naive (29.51%, 36/122). The top five dominant genera of the total population (122 cases) were Prevotella, Roseburia, Megamonas, Bacteroides and Faecalibacterium and the top five dominant genera of the ART population (86 cases) were Prevotella, Megamonas, Bacteroides, Roseburia and Faecalibacterium. The top five dominant genera of the ART-naive population (36 cases) appeared as Prevotella, Faecalibacterium, Roseburia, Bacteroides and Megamonas. In the total population, ART (P<0.001) was the most significant factors of community structure. Other significant factors were:duration of diagnosis (P=0.009), viral load (P=0.022) and anti-HCV (P=0.018). ART was positively correlated with Megamonas and negatively correlated with Prevotella,Roseburia and Faecalibacterium, while the other three factors of duration of diagnosis, viral load and anti-HCV were positively correlated with Prevotella,Roseburia and Faecalibacterium and negatively correlated with Megamonas. In the ART-naive population, duration of diagnosis (P=0.003) were the factors significantly associated with community structure. Duration of diagnosis was positively correlated with Roseburia, Faecalibacterium,Megamonas and Prevotella and negatively correlated with Bacteroides.Conclusion ART and duration of diagnosis were factors significantly associated with gut microbial community structure and had a significant impact on multiple high-abundance genera.
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