文章摘要
董莉娟,陈会超,马艳玲,邢文革.HIV-1 DNA的检测及临床应用[J].中华流行病学杂志,2022,43(10):1685-1690
HIV-1 DNA的检测及临床应用
Detection and clinical application of HIV-1 DNA
收稿日期:2021-12-30  出版日期:2022-10-18
DOI:10.3760/cma.j.cn112338-20211230-01036
中文关键词: 艾滋病病毒  脱氧核糖核酸  检测  临床应用
英文关键词: HIV  DNA  Detection  Clinical application
基金项目:
作者单位E-mail
董莉娟 云南省疾病预防控制中心性病艾滋病防制所, 昆明 650022  
陈会超 云南省疾病预防控制中心性病艾滋病防制所, 昆明 650022  
马艳玲 云南省疾病预防控制中心性病艾滋病防制所, 昆明 650022 mayanling68@aliyun.com 
邢文革 中国疾病预防控制中心性病艾滋病预防控制中心参比实验室, 北京 102206 xingwenge@sina.com 
摘要点击次数: 1521
全文下载次数: 911
中文摘要:
      HIV-1储存库的持续存在是治愈HIV的主要障碍,在临床研究中,需要可靠的生物标志物对其进行标记。HIV-1 DNA在HIV-1储存库中可被持续检测到,在HIV-1感染诊断、预测病毒反弹和监测治疗效果等方面具有重要应用价值。PCR的检测技术是临床上常用的HIV-1 DNA检测方法,随着技术的不断创新与进步,可更准确地通过定性或定量检测感染细胞中总的、整合的和未整合的HIV-1 DNA。感染细胞中不同形式的HIV-1 DNA作为生物标志物在HIV感染监测和艾滋病治疗相关研究中报道日益增多。本文对感染细胞中HIV-1 DNA的检测方法及其作为生物标志物的临床应用进展进行综述。
英文摘要:
      The persistence of the HIV-1 reservoir is still the main obstacle to the cure of HIV. In clinical research, reliable biomarkers are needed to label it. HIV-1 DNA can be continuously detected in the HIV-1 reservoir. It has significant application value in diagnosing HIV-1 infection, the timing of antiretroviral therapy, the prediction of virus rebound, and monitoring treatment effects. The detection technology based on polymerase chain reaction (PCR) is the most commonly used HIV-1 DNA detection method in clinical practice. The continuous innovation and advancement of technology can accurately detect the total, integrated, and unintegrated HIV-1 DNA in infected cells using qualitative or quantitative methods. Different forms of HIV-1 DNA in infected cells have been increasingly reported as biomarkers in HIV infection monitoring and AIDS treatment-related research. This article reviews the progress of HIV-1 DNA
查看全文   Html全文     查看/发表评论  下载PDF阅读器
关闭