文章摘要
郭晓琪,张爽,郑徽,王锋,缪宁,苏秋东,毕胜利,张国民,王富珍,沈立萍.中国15个民族HBV基因型及基因亚型分布特征分析[J].中华流行病学杂志,2023,44(5):759-764
中国15个民族HBV基因型及基因亚型分布特征分析
Epidemiological distribution of genotypes and sub-genotypes of hepatitis B virus in 15 ethnic groups in China
收稿日期:2022-11-30  出版日期:2023-05-13
DOI:10.3760/cma.j.cn112338-20221130-01021
中文关键词: 乙型肝炎病毒  基因型  基因亚型  民族分布
英文关键词: Hepatitis B virus  Genotype  Sub-genotype  Ethnic group distribution
基金项目:国家科技重大专项(2017ZX10105015-001-002)
作者单位E-mail
郭晓琪 中国疾病预防控制中心病毒病预防控制所, 北京 102206  
张爽 中国疾病预防控制中心病毒病预防控制所, 北京 102206  
郑徽 中国疾病预防控制中心免疫规划中心, 北京 100050  
王锋 中国疾病预防控制中心病毒病预防控制所, 北京 102206  
缪宁 中国疾病预防控制中心免疫规划中心, 北京 100050  
苏秋东 中国疾病预防控制中心病毒病预防控制所, 北京 102206  
毕胜利 中国疾病预防控制中心病毒病预防控制所, 北京 102206  
张国民 中国疾病预防控制中心免疫规划中心, 北京 100050  
王富珍 中国疾病预防控制中心免疫规划中心, 北京 100050 wangfz@chinacdc.cn 
沈立萍 中国疾病预防控制中心病毒病预防控制所, 北京 102206 shenlp@ivdc.chinacdc.cn 
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中文摘要:
      目的 了解我国不同民族人群感染HBV的基因型及基因亚型分布特征。方法 利用多阶段分层整群随机抽样结合系统抽样的方法从2020年全国乙型肝炎血清流行病学调查HBsAg阳性样本库中抽取样本,利用巢式PCR扩增阳性样本HBV S区基因,构建系统发育树分析判定HBV基因型和亚型,结合社会人口学资料进行综合分析。结果 成功扩增15个民族的1 539份样本,检出B、C、D、I型和C/D重组型5种基因型。B型占比较高的民族包括汉(74.52%,623/836)、壮(49.28%,34/69)、彝(53.19%,25/47)、苗(94.12%,32/34)和布依族(81.48%,22/27);C型占比较高的民族为瑶族(70.91%,39/55);D型占比较高的民族为维吾尔族(83.78%,31/37);C/D重组型占比较高的民族为藏族(92.35%,326/353);检出I型11例中,8例来自于壮族。除藏族外,各民族的B型中B2亚型均>80.00%;在C型中,C2亚型占比较高的民族包括汉、藏、彝、维吾尔、蒙古、满、回和苗族8个民族,C5亚型占比较高的民族包括壮(55.56%,15/27)和瑶族(84.62%,33/39);在D型中,彝族均为D3亚型,维吾尔和哈萨克族均为D1亚型。C/D1和C/D2亚型在藏族占比分别为43.06%(152/353)和49.29%(174/353)。I型均为I1亚型。结论 我国15个民族中发现HBV 5种基因型和15种基因亚型,不同民族的HBV基因型和基因亚型分布差异明显。
英文摘要:
      Objective To understand the distribution of genotypes and sub-genotypes of HBV in different ethnic groups in China. Methods The HBsAg positive samples were selected by stratified multi-stage cluster sampling from the sample base of national HBV sero-epidemiological survey in 2020 for the amplification of S gene of HBV by nested PCR. A phylogeny tree was constructed to determine the genotypes and sub-genotypes of HBV. The distribution of genotypes and sub-genotypes of HBV were analyzed comprehensively by using laboratory data and demographic data. Results A total of 1 539 positive samples from 15 ethnic groups were successfully amplified and analyzed, and 5 genotypes (B, C, D, I and C/D) were detected. The proportion of genotype B was higher in ethnic group of Han (74.52%, 623/836), Zhuang (49.28%, 34/69), Yi (53.19%, 25/47), Miao (94.12%, 32/34), Buyi (81.48%, 22/27). The proportions of genotype C were higher in ethnic groups of Yao (70.91%, 39/55). Genotype D was the predominant genotype in Uygur (83.78%, 31/37). Genotype C/D were detected in Tibetan (92.35%,326/353). In this study, 11 cases of genotype I were detected, 8 of which were distributed in Zhuang nationality. Except for Tibetan, sub-genotype B2 accounted for more than 80.00% in genotype B in all ethnic groups. The proportions of sub-genotype C2 were higher in 8 ethnic groups, i.e. Han, Tibetan, Yi, Uygur, Mongolian, Manchu, Hui and Miao. The proportions of sub-genotype C5 were higher in ethnic groups of Zhuang (55.56%, 15/27) and Yao (84.62%, 33/39). For genotype D, sub-genotype D3 was detected in Yi ethnic group and sub-genotype D1 was detected in both Uygur and Kazak. The proportions of sub-genotype C/D1 and C/D2 in Tibetan were 43.06% (152/353) and 49.29% (174/353). For all the 11 cases of genotype I infection, only sub-genotype I1 was detected.Conclusions Five genotypes and 15 sub-genotypes of HBV were found in 15 ethnic groups. There were significant differences in the distribution of genotypes and sub-genotypes of HBV among different ethnic groups.
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