| 樊武舫.酶联免疫吸附试验检测森林脑炎IgG抗体的研究[J].Chinese journal of Epidemiology,1984,5(2):107-110 |
| 酶联免疫吸附试验检测森林脑炎IgG抗体的研究 |
| A Study on the Application of ELISA to Detect IgG-antibody Against Spring-Summer Encephalitis |
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| Abstract: |
| 本文报告了用ELISA技术检测森脑病毒IgG抗体的实验条件和方法，并与HIT和CFT作了比较。抗原由感染鼠脑经鱼精蛋白处理-聚乙二醇浓缩及蔗糖密度梯度离心纯化制备。共检测了109份血清，结果发现ELISA与HIT的检出符合率为93.6%，与CFT的检出符合率为86.2%；ELISA与HIT和CFT的相应检出滴度相关非常显著（rHIT=0.9550，rCFT=0.8912，P<0.001），且比HIT和CFT分别敏感10~80倍及50~200倍，未发现与乙脑免疫血清存在交叉反应。ELISA技术特异性和重复性均好，所需标本量少且不需预先处理，可代替常规方法用于森脑的临床诊断、血清流行病学调查等研究。 |
| English Abstract: |
| A method was developed to use ELISA for detction of IgGantibody against spring-summer ence-halitis, compared with HIT and CFT.Antigen was repared from infected mice brains treated with protamine,PEG and purified with sucrose gradient ultracentrifugation.109 samples of sera were examined It was found that the agreement between ELISA and HIT was 93.6%,ELISA and CFT 86.2%.The correlation between their titres was very significant (rHIT=0.9550, rCFT=0.8912, P<0.001). ELISA proved 10-80 times more sensitive than HIT and 50-200 times than CFT.Unspecific cross reaction with Japanese Encephalitis had not been found.In view of its specificity, high reproducibility and requirment of only small amount of sample,ELISA was recom-mended asa routine in clinical and epidemiological investigation of spring-summer encephalitis. |
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