徐志一,法金生,任世玲,刘春荣.固相酶标记双夹心法检测抗HBc-IgM[J].Chinese journal of Epidemiology,1984,5(3):176-179 |
固相酶标记双夹心法检测抗HBc-IgM |
A Solid-phase Double-sandwich ELISA for Detecting Anti-HBc IgM |
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Abstract: |
本文报告一种酶联免疫法,用抗μ包被的聚苯乙烯微孔塑料板检测抗HBc-IgM。10份抗HBc阳性正常人血清与6份类风湿阳性血清均未显示假阳性。113例急性肝炎中,42例抗HBc-IgM阳性,51例抗HAV IgM阳性。分别判为甲、乙型肝炎。20例两种IgM均阴性,其中19例判为非甲非乙型,1例抗HBs阳转判为乙肝。两种IgM检测的阳性结果未发现交叉。10名HBsAg携带者中有4人以及11例慢性肝炎中有10例显示抗HBc-IgM阳性。1例慢活肝病人抗HBc-IgM于复发前一周由10-4上升至10-6,乙肝病人抗HBc-IgM滴度于病初即达10-6。本试验灵敏度与特异性均佳;可区别近期感染和既往感染,但不能区别急性与慢性乙肝感染。早期清除HBsAg的乙肝病人及重叠感染甲型或非甲非乙型肝炎的HBsAg携带者,不能用HBsAg的指标来诊断,而只能用本试验来确定。 |
English Abstract: |
An enzyme-immunoassay was developed/using the IgM capture procedure with anti-μ coated poly-sterene microplates. No false-positive results were detected in 10 normal human sera positive for anti-HBc and 6 sera positive for RF. Among 113 acute hepatitis cases, anti-HBc IgM was detected in 42 cases, anti-HAV IgM was detected in 51 cases and they were identified as hepatitis A and B, respectively. No specific IgM to HAV or to HBcAg were detected in the other 20 cases. Nineteen of them were identified as non-A non-B with one anti-HBs conversion later considered to be hepatitis B. No cross positivity was observed between tests for the two specific IgM. In the tests,4 out of 10 HBsAg carriers and 10 out of 11 patients with chronic hepatitis sh-owedanti-HBc IgM.The titer of anti-HBcIgM reached 10-6 in the early stage of acute hepatitis B. In one case withchronic active hepatitis, the titer rised from 10-4 to 10-6 in one week, preceding the recurrence of hepatitis. The test is highly sensitive and specific. It is useful for differentiating the recent Past and current hepatitis B infections from remote infection, but it can not be used in differentiating the carrier state and chronic hepatitis B from the acute one. Patients with acute hepatitis B who cleared HBsAg at an early stage, and HBsAg carriers with superimposed hepatitis A or non-A non-B infection might be misdiagnosed by the test for HBs Ag alone.The patients and carriers can be correctly defined only by the tests for specific IgM to HAV and to HBcAg. |
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