| 陈永祥,王光明,方喜业.微量间接血凝法(单醛固定)及其诊断弓形体病的实验观察[J].Chinese journal of Epidemiology,1985,6(1):45-46 |
| 微量间接血凝法(单醛固定)及其诊断弓形体病的实验观察 |
| Experimental observations on the diagnostie valuo of Indirect Haemagglutination Miero technigue (With Glutaric dialdehyde fixation) used in detecting Human Toxoplasmosis |
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| Abstract: |
| 本文应用单醛法固定绵羊红细胞,经鞣酸鞣化和抗原敏化后做弓形体间接血凝调查人群弓形体抗体。同时与常用的双醛法作比较,结果相同。以此法对北京市人群血清作弓形体病血清学调查时,在571份血清中检出57份阳性,阳性率为9.98%,其中有病历可查的191份中有35份阳性。在这些阳性者中,经过卡方检验,只有性别组有显著性差异(男高于女)。其他各组均无差异。 |
| English Abstract: |
An indirect hemagglutination micro test was applied to detect the presence of antibodies against toxoplasma in. human sera by means of sensitized sheep erythrocyte after glutaric dialdehyde fixation and tannic acid treatment.
This procedure has been compared with that of methyl glyoxal and formaldehyde method and was found to yield similar resucts and quite reliable.
The survey was undertaken in certain regions of Beijing.Among 571 samples of tested sera, 57 were found to be positive with a rate of 9.98%. Among selected 191 sera obtained from different kinds of patients revealed 35 positive. The statistical analysis showed that only sex group presented remarkable dif ference, i.e.positive rate in male was higher than in female.There is no correlation between positive rates to ages and obviously farmers and workers are those possessing higher positive rates. |
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