IgM抗体捕捉ELISA法在麻疹血清学诊断上的优越性

郭可謇; 宋光运; 张礼璧; 张荣珍; 郑锡茹; 武士珍; 王凝芳

Abstract

郭可謇,宋光运,张礼璧,张荣珍,郑锡茹,武士珍,王凝芳.IgM抗体捕捉ELISA法在麻疹血清学诊断上的优越性[J].Chinese journal of Epidemiology,1986,7(6):352-355

IgM抗体捕捉ELISA法在麻疹血清学诊断上的优越性

The Advantages of IgM Antibody Capture ELISA for Measles Diagnosis

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Author Name	Affiliation
Guo Kejian	Institute of Virology, Chinese Academy of preventive Medicine, Beijing. etc.
宋光运	Institute of Virology, Chinese Academy of preventive Medicine, Beijing. etc.
张礼璧	Institute of Virology, Chinese Academy of preventive Medicine, Beijing. etc.
张荣珍	中国预防医学科学院流行病学微生物学研究所
郑锡茹	中国预防医学科学院流行病学微生物学研究所
武士珍	中国预防医学科学院流行病学微生物学研究所
王凝芳	中国人民解放军302医院

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Abstract:

本文用ACELISA法、ELISA-IgG法、HI法测定了临床诊断为麻疹的100对患者双份血清,其中8份三种方法都为阴性。余92对血清的三种方法诊断阳性率分别为ACELISA法98.9%,ELISA-IgG法82.6%,HI法69.4%,证明ACELISA法是诊断麻疹较好的方法,有敏感、特异、早期、多数仅需单份血清就能诊断等的优点。IgM抗体发病第二天开始出现,病后第2周达到高峰,一个月后明显下降或阴转,故ACELISA法诊断的适宜采血时期为发病第3~25天。

English Abstract:

100 paired sera from measles patients diagnosed by clinics were tested for further confirmation by IgM antibody capture ELISA (ACELISA), IgG-ELISA and HI tests.From them 8 cases showing negative results in their paired sera by all the three tests were considered not to be suffered from measles infection. The other 92 pairs of sera sho wed indices of recent measles infection by one to three of these tests and the positive diagnostic rates were different by each test, i.e. IgM-ACE LISA 98.9%, IgG-ELISA 82.6%, HI 69.4%. The AC ELISA was proved to be the best method for measles diagnosis with the advantages of sensitivity, specificity and earlier diagnosis.
Measles IgM antibody starts at the 2nd day of illness and goes to its peak by about the 2nd week. It decreases and begins to convert to negative by one month after onset of the disease. Thus, the optimal blood collecting time for measles diagnosis by ACELISA is considered approximately from the 3rd to the 25th day of illness.

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