| 黄文洲,罗曼珍.醋酸纤维膜免疫扩散法鉴定蚊胃血血源的研究[J].Chinese journal of Epidemiology,1987,8(1):43-45 |
| 醋酸纤维膜免疫扩散法鉴定蚊胃血血源的研究 |
| A Cellulose Acetate Membrane Immunodiffusion (CAMID) Test for Identification of Host Source of Mosquito Blood Meals |
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| Abstract: |
| 以醋釺膜双向免疫扩散法鉴定蚊胃血血源,经试验选定的工作浓度抗血清为1:2及抗原为1:500,于37℃中免疫扩散2.5小时后观察结果,与常用的环状沉淀试验法同时对比鉴定中华按蚊胃血标本,两法结果一致及特异性相似,表明这是鉴定蚊胃血另一较为快速的免疫扩散方法。 |
| English Abstract: |
A double lmmunodiffusion test using cellulose acetate membrane has been tried to identify the host source of mosquito blood meals and compared with the precipitin ring test (PRT). The optimum dilutions of antiserum and antigen for the test are 1:2 and 1:500 respectively. After an incubation for 2.5 hours at 37℃ a positive result was shown by presence of a precipitin arc between the serum and the antigen.
72 and 36 blood meal specimens from Anopheles sinensis fed through a membrane on fresh human or pig blood and 1,061 specimens collected from field and 96, 36 and 16 specimens stored for 6, 16 and 29 months at 4℃ respectively were examined. The results of the two methods were in good agreement. In addition, positive rates of 99.8% and 69.3% were observed in 452 specimens collected from field and test by CAMID and agar gel diffusion (AGD) method respectively. It appears to be that the accuracy and the specificity between PRT and CAMID or AGD and CAMID methods for identification of mosquito blood meals were identical. |
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