聚丙烯酰胺凝胶电泳(PAGE)检测肠道腺病毒的实验研究

陈元鼎; 戴国珍; 李军; 丁雪凤; 孙茂盛; 王丽春; 徐维明; 苏晔; 杜丹萍; 郭仁

Abstract

陈元鼎,戴国珍,李军,丁雪凤,孙茂盛,王丽春,徐维明,苏晔,杜丹萍,郭仁.聚丙烯酰胺凝胶电泳(PAGE)检测肠道腺病毒的实验研究[J].Chinese journal of Epidemiology,1989,10(6):366-368,401

聚丙烯酰胺凝胶电泳(PAGE)检测肠道腺病毒的实验研究

Polyacrylamide Gel Electrophoresis (PA GE) as a Method for Detecting Enteric Adenovirus (EAd)

DOI：

KeyWord: PAGE 肠道腺病毒轮状病毒呼肠孤病毒

English Key Word: PAGE EAd RV ReoV

FundProject:

Author Name	Affiliation
Chen Yuan-ding	Institute of Medical Biology, Chinese Academy of Medical Sciences
戴国珍	中国医学科学院医学生物学研究所世界卫生组织肠道病毒参考和研究合作中心（现在广州市卫生防疫站）
李军	Institute of Medical Biology, Chinese Academy of Medical Sciences
丁雪凤	Institute of Medical Biology, Chinese Academy of Medical Sciences
孙茂盛	Institute of Medical Biology, Chinese Academy of Medical Sciences
王丽春	Institute of Medical Biology, Chinese Academy of Medical Sciences
徐维明	Institute of Medical Biology, Chinese Academy of Medical Sciences
苏晔	Institute of Medical Biology, Chinese Academy of Medical Sciences
杜丹萍	Institute of Medical Biology, Chinese Academy of Medical Sciences
郭仁	Institute of Medical Biology, Chinese Academy of Medical Sciences

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Abstract:

本文报道运用聚丙烯酰胺凝胶电泳(PAGE)技术同时检测肠道腺病毒(EAd)、轮状病毒(RV)和呼肠孤病毒(ReoV)及非肠道腺病毒(NEAd)的研究结果。并用PAGE检测技术与电镜技术(EM)进行了比较研究。结果表明,PAGE是检测肠道腺病毒的简便、快速、可靠的技术,并可同时检出轮状病毒和呼肠孤病毒,它与EM的符合率为94%,适合于实验室和临床常规的流行病学研究。

English Abstract:

This paper presents a method which could provide a simple, rapid, economical, and reliable means of detecting or identifying adenoviruses (Advs), rotaviruses (RVs) and reeviruses (ReoVs) in stool suspensions or tissue cultures. The method is based on polyacrylamide gel electrophoresis (PAGE) of the virus nucleic acids, but sample preparation does not need the use of radioactive label, specific DNA probe or antisera.Comparison of the results of PAGE of Adv, RV and ReoV with those of electron microscopy (EM) and/or enzyme-linked immunoabsorbent assay (ELISA) was made.The method is of comparable sensitivity to electron microscopy, and is not limited by amount of specimens obtained, and is thus suitable for application as a batch testing method.

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