| 尚德秋,鲁齐发,武素怀,李元凯,李兰玉,姜淑贤,程尧章,崔春槐.布鲁氏菌非典型菌株及R型菌株鉴定分类的研究[J].Chinese journal of Epidemiology,1990,11(3):160-166 |
| 布鲁氏菌非典型菌株及R型菌株鉴定分类的研究 |
| A Study on Identification of Atypical and R Phase Strains of Brucella |
| Received:March 13, 1989 Revised:June 15, 1989 |
| DOI： |
| KeyWord: 布备氏菌 非典型菌株 |
| English Key Word: Brucella Atypical stiains |
| FundProject: |
| Author Name | Affiliation | | Shang De-qui | Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing | | 鲁齐发 | Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing | | 武素怀 | Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing | | 李元凯 | Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing | | 李兰玉 | Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing | | 姜淑贤 | Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing | | 程尧章 | Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing | | 崔春槐 | Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing |
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| Abstract: |
本文报告了用布氏菌常规鉴定法、六大群噬菌体裂解试验和钒化代谢捡查对17株非典型及R型菌株进行综合鉴定分类研究;并建立和采用4株布氏菌系列McAb和S型牛、羊、猪种布氏菌的OMP的SDS-PAGE图谱用于非典型布氏菌分类之中。
通过实际鉴定菌株证明,采用常规鉴定方法、六大群噬菌体裂解分甩和氧化代谢试验的综合鉴定可以对大多数非典型和R型菌株定种定型。
首次对利用布氏菌系列McAb和布氏菌OMP图谱鉴定非典型及R型菌株进行探索,并为利用这两种方法鉴定这两类菌株的可能性提供了依据。 |
| English Abstract: |
This paper reports a comprehensive study on taxonomy of Brucella. In which 17 atypical and R phase strains were identified by using the routine methods, 6 goups of phages and oxidative metabolic tests. 4 McAb of Brucella were developed and applied for identification.Meanwhile OMP profiles of S phase of B. abortus, B. melitensis and B. suis were compared during identification of them.
The results show that a comprehensive identification with routine methods, phagetyping and oxidative metabolic tests arc able to determine these strains if they are species or biotypes.
Several strains of Brucella McAb and OMP profiles of Brucella were firstly applied for identification of atypical and R strains, and provided some possibility in the applica-tion of identification of Brucella by using these two methods. |
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