| 左曙生,李钟铎.时间分辨免疫荧光技术在病毒检测中的应用[J].Chinese journal of Epidemiology,1993,14(6):362-365 |
| 时间分辨免疫荧光技术在病毒检测中的应用 |
| Application of the Time-resolved Immunofluorometric Assay in the Detection of Viruses |
| Received:January 20, 1993 Revised:June 21, 1993 |
| DOI： |
| KeyWord: 时间分辨免疫荧光技术 病毒 |
| English Key Word: Time-resolved immunofluorometric assay Viruses |
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| Abstract: |
| 目前,时间分辨免疫荧光技术(TR-IFMA)已有的两大系统即LKB系统和Cyberfluor系统均已应用于病毒的检测,主要检测的病毒有腺病毒、流感病毒、副流感病毒、呼吸道合胞病毒,轮状病毒、乙肝病毒、艾滋病毒、风疹病毒等,与酶联、放免等其它方法相比,TR-IFMA具有敏感性高,特异性强,临床符合率高等优点,TR-IFMA检出病毒抗原的最低值在10~100pg之间,双标记TR-IFMA的应用也具有较好的效果,TR-IFMA还可用于病毒核酸的检测,其检出的靶区域可达pg水平。 |
| English Abstract: |
| Two systems of time-resolved immunofluorometric assay (TR-IFMA), i.e., LKB system and Cyberfluorsystem, were applied in the detection of viruses. The viruses detected were mainly adenovirus, influenza virus, parainfluenza virus, respiratory syncytial virus, rotavirus, hepatitis B virus, human immunodeficiency virus, rubella virus, etc. Compared with methods of ELISA and RIA, TR-IFMA has the merits of high sensitivity, specificity and consistency with clinical diagnosis. The low detection limit of virus antigen by TR-IFMA is between 10~100pg. The application of duallabel TR-IFMA also showed good effect. TR-IFMA can also be used in the detection of viral nucleic acids. The target region detected may reach the level of pg. |
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