| 刘沛,舒华嵩,张言超,鲍勇,丁莹,张新英.混合血清法筛检丙型肝炎抗体可行性的血清流行病学研究[J].Chinese journal of Epidemiology,1994,15(3):179-181 |
| 混合血清法筛检丙型肝炎抗体可行性的血清流行病学研究 |
| Seroepidemiologic Study on the Feasibility of Screening for the Antibody to HCV by Pooling the Serum Specimens |
| Received:April 06, 1993 Revised:May 28, 1993 |
| DOI： |
| KeyWord: 混合血清法 抗-HCV筛检 酶免疫法 |
| English Key Word: Serum Pooling Method Screening for Antibody to HCV EIA |
| FundProject:本研究由徐州市科学技术委员会，徐州医学院资助 |
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| Abstract: |
| 应用单盲随机化控制的流行病学方法研究了10个血清标本混合,筛检抗-HCV的可行性。为保证每个标本加样量及每孔液体总量与EIA要求相同,只改变被测混合标本同所加标本稀释剂之间量的比例,其它操作方法与试剂盒说明书一致。结果显示:以逐个检验法为对照,混合血清法的灵敏度为100%,特异度为97.6%,假阳性率为2.4%,Kappa值为0.95(P<0.05)。收益分析发现,应用混合血清法筛检抗-HCV可获得显著的经济效益。作者认为,在用EIA检测抗-HCV的实验中,将10个标本混合进行抗-HCV筛检是可行的。 |
| English Abstract: |
| The feasibility of screening for the antibody to HCV by pooling 10 serum specimens was studied by utilizing the epidemiologic method of blind randomized controlled trial. We changed only the ratio between the pooled serum specimen volume and the serum specimen diluent volume, with their total volume and single serum specium volume being as much as the volume required by EIA. Other steps were the same as those of EIA. The result showed that taking single serum specimen as the control group, the sensitivity and specificity of pooled serum specimen were 100% and 97.6%, respectively. The false positive rate was 2.4%, Kappa value was 0.95 (P<0.05). The analysis of profit found that remarkable social and economic benefit will be obtained when serum pooling method is used. These results demonstrate that pooling 10 serum specimens is feasible to screening the antibody to HCV in blood donors by EIA. |
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