酶免疫斑点试验用于钩端螺旋体病早期血清学诊断的研究

林涛; 时曼华; 龙健; 聂一新

Abstract

林涛,时曼华,龙健,聂一新.酶免疫斑点试验用于钩端螺旋体病早期血清学诊断的研究[J].Chinese journal of Epidemiology,1994,15(4):223-226

酶免疫斑点试验用于钩端螺旋体病早期血清学诊断的研究

Studies on the Early Serodiagnosis of Leptospirosis by dot-ELISA

Received:October 21, 1993 Revised:December 06, 1994

DOI：

KeyWord: 钩端螺旋体病酶免疫斑点试验显微镜凝集试验血培养

English Key Word: Leptospirosis Dot-ELISA MAT Blood culture

FundProject:

Author Name	Affiliation
Lin Tao	Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing 102206
Shi Manhua	Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing 102206
Long Jian	江西省上高县卫生防疫站
聂一新	Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing 102206

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Abstract:

应用酶免疫斑点试验(dot-ELISA)和显微镜凝集试验(MAT)对培养钩端螺旋体(钩体)阳性的30份早期钩端螺旋体病(钩体病)人血清进行了检测,发现dot-ELISA的阳性率(40%)明显高于MAT的阳性率(13.3%)。同时对198份临床确诊为早期钩体病人的血清进行了dot-ELISA、MAT和血培养对照研究,证明dot-ELISA的阳性率(64.6%)也明显高于血培养阳性率(15.2%),同时也高于MAT的阳性率(14.1%)。随着发病时间的延长,血培养阳性率逐渐降低,dot-ELISA阳性率变化不明显。

English Abstract:

Thirty serum samples of patients with early leptospirosis from whom cultures of leptospira were positive were tested by dotenzyme linked immunosorbent assay (dot-ELISA) and microscopical agglutination test (MAT). The results showed that the positive rate of dot-ELISA (40%) was higher than that of MAT (13.3%). One-hundred and ninety-eight serum samples from patients with clinical diagnosis of early leptospirosis were tested by dot-ELISA, MAT and blood culture. The results showed that the positive rate of dot-ELISA (64.6%) was higher than that of blood culture (15.2%) and MAT (14.1%). The longer the time of the illness, the lower the positive rate of the blood culture. However, the positive rate of dot-ELISA was stable in different stages of the illness.

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