应用VIDAS免疫分析系统检测血清抗伯氏包柔螺旋体抗体

关淑珍; 尹国才; 韩善桥; 化冰; 刘群英

Abstract

关淑珍,尹国才,韩善桥,化冰,刘群英.应用VIDAS免疫分析系统检测血清抗伯氏包柔螺旋体抗体[J].Chinese journal of Epidemiology,1994,15(6):368-371

应用VIDAS免疫分析系统检测血清抗伯氏包柔螺旋体抗体

Detection of Anti-BB Antibodies in Human Sera by VIDAS System

Received:October 06, 1993 Revised:February 22, 1994

DOI：

KeyWord: 莱姆病（Lyme病）抗BB抗体 VIDAS系统 IFA

English Key Word: Lyme disease Anti-BB antibodies VIDAS System IFA

FundProject:

Author Name	Affiliation
Guan Shuzhen	Department of Laboratory. Navy General Hospital. Beijing 100037
Yin Guocai	Department of Laboratory. Navy General Hospital. Beijing 100037
Han Shanqiao	Department of Laboratory. Navy General Hospital. Beijing 100037
化冰	Department of Laboratory. Navy General Hospital. Beijing 100037
刘群英	Department of Laboratory. Navy General Hospital. Beijing 100037

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Abstract:

莱姆病(Lyme disease)是最近十年来才被认识的一种蜱媒传染性流行病。Lyme病的血清学诊断方法有间接荧光免疫法(IFA),酶免疫试验(EIA),免疫印迹法(WB)。梅里埃生物公司(Biomerieux)推出VIDAS免疫诊断系统能够自动定性和半定量地检查Lyme病的抗伯氏包柔螺旋体抗体(抗BB抗体)。本法结合了酶和荧光免疫两种方法。硷性磷酸酶标记二抗的底物是4methylumbellifery phosphate,底物在碱性磷酸酶的催化下转变成4-methylumbelliferon,该产物在365nm光激发下可发出455nm的荧光,光强度被VIDAS中的光扫描器测定出来。我们用VIDAS检测了体检标本83例,阳性8份(9.6%);检测临床为结核病的标本20份,无一例阳性;临床诊断为结节病或可疑结节病标本46份,阳性4份(8.7%);各种眼科疾病57份,阳性13份(22.8%);口唇疾病15份,阳性4份(26.7%);其他标本17份,阳性2份(11.8%);检测了军事医学科学院(军科院)微生物流行病研究所采自新疆、黑龙江省可疑Lyme病病人的标本16份,检出阳性12份(75.0%)。
笔者用VIDAS系统和IFA方法比较了165份标本,VIDAS检出阳性24份(14.5%),IFA法检出131份(79.4%),两法一致性为32.7%。

English Abstract:

Lyme disease is an infectious disease just known in the latest decade. The sera of people from various origins were detected for Anti-BB antibodies (IgM+IgG) by VIDAS system. The results are as follows:Of 83 sera from the health-cared people, 8 were positive (9.6%); of 20 sera from the patients clinical diagnosised as tuberculosis, none was positive; of 46 sera from the patients clinical diagnosised or suspected as node diseasas, 4 were positive (8.7%); of 57 sera from the patients with various ophthalmologic diseases, 13 were positive (22.8%); of 15 sera from the patients with stomatologic diseases, 4 were positive (26.7%); of 17 sera from the patients with various diseases including dermomyositis, liver and spleen swelling, undefinited febrile diseases; lung cancer, lung shadow, etc.), 2 were positive (11.8%); of 16 sera from the people highly suspected with lyme disease living in the epidemic areas of Xin Jiang and Hei Long Jiang provinces, 12 were positive (75%).
The same 165 sera samples were detected at the same time by VIDAS System and IFA, 24 were positive by VIDAS System (14.5%), and 131 positive by IFA (79%), the accordance rate was only 32.7%.

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