| 郭增柱,祝宏,安亦军,王正仪.多聚酶链反应在阿米巴病流行病学研究中的应用[J].Chinese journal of Epidemiology,1995,16(5):303-305 |
| 多聚酶链反应在阿米巴病流行病学研究中的应用 |
| Application of the Polymerase Chain Reaction to the Epidemiological Study of Amoebiasis |
| Received:November 20, 1994 Revised:February 14, 1995 |
| DOI： |
| KeyWord: 阿米巴病 溶组织内阿米巴 多聚酶链反应 流行病学调查 |
| English Key Word: Amoebiasis Entamoeba histolytica PCR Epidemiological study |
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| Abstract: |
| 用两对引物扩增(PCR)溶组织内阿米巴带囊者粪便中的DNA,以鉴别感染虫株的致病性。32例中,致病和非致病性PCR均为阳性者1人(3.1%),仅一项阳性者分别为6人(18.8%)和25人(78.1%)。7例致病性PCR阳性者中,6人有腹泻症状(OR=31.5)。各种对照标本均呈PCR阴性反应。以PCR法对一小样本人群进行阿米巴病调查,致病和非致病虫株的感染率均为10%。结果表明,在阿米巴病流行病学调查中,可应用PCR鉴别感染虫株的致病性,具有简便快速等优点。 |
| English Abstract: |
| The polymerase chain reaction (PCR) has been used in studying the pathogenicity of Entamoeba histolytica in a rural community in Shandong province. Formalin-fixed stool samples were used for extraction of DNA. The PCR amplifications were performed using two sets of primer that discriminate between pathogenic and nonpathogenic E. histolytica. All of the 32 cases of cyst carrier diagnosed microscopically were identified as positive. Three groups were defined through PCR analysis:6 cases (18.8%) were positive for pathogenic but negative for nonpathogenic, 25 cases (78.1%) were negative for pathogenic but positive for nonpathogenic, and only one case (3.1%) was positive for both pathogenic and nonpathogenic. The cases found to be pathogenic by PCR were also closely correlated with their clinical manifestations (diarrhea) (OR=31.5,P<0.005). All of the control cases showed negative reaction to PCR. PCR has also been used to study the epidemiology of amoebiasis in a small population group with 40 persons in the same province. Both positive rates by PCR on pathogenic and nonpathogenic were 10%. The results showed that this new technique can be applied in the field for the epidemiological studies of pathogenic and nonpathogenic E. histolytica. |
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