| 陈晶晶,杨昭徐,蒋秀高,张建中,梁丕霞.幽门螺杆菌全菌抗原与尿素酶纯化抗原ELISA检测在人群血清学诊断中的应用[J].Chinese journal of Epidemiology,1996,17(1):44-46 |
| 幽门螺杆菌全菌抗原与尿素酶纯化抗原ELISA检测在人群血清学诊断中的应用 |
| A Comparison of Purified Urease Antigen and Whole Cell Antigen of Helicobacter pylori by ELISA Test-Study on the Application and Serum Diagnoses of Helicobacter pylori Urease Diagnostic Reagent |
| Received:September 05, 1995 Revised:October 08, 1995 |
| DOI： |
| KeyWord: 幽门螺杆菌 尿素酶 酶联免疫法 |
| English Key Word: Helicobacter pylori Urease Enzyme linked immunsorbent assay (ELISA) |
| FundProject:本课题为国家自然科学基金资助项目 |
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| Abstract: |
| 用纯化的Hp抗原对676例各型胃病患者进行了血清抗幽门螺杆菌尿素酶抗体的检测。实验证明其特异性为96%,敏感性为98%。血清学ELISA检测法,尤其适宜于流行病学调查,还可用于药物治疗效果的观察和筛选非溃疡性消化不良。 |
| English Abstract: |
| A useful assay for epidemiologieal survey of H. pylon infection was reported, using the urease antigen of H. pylon to detect the anti-urease antibody in sera from 676 patients suffered from gastropathy with ELISA technique, and compared with whole cell antigen. Results showed that the purified urease antigen was better than whole cell antigen. The partially purified urease antigens rapid diagnostic reagent of H. pylon was examined in hospital/institution and compared with whole-cell antigens. Results of sera from 676 H. pylon-positive gastritis and non-ulcer dyspepsia patients being tested showed that specificity and sensitivity of ELISA were 96% and 98% respectively. It seemed to be very useful for epidemiological studies on H. pylon infection. The use of ELISA in the detection of IgG antibodies against H. pylon was also sensitive, specific and rapid in assessing the improvement of both acute and chronic inflammation,cleaning of bacteria and the antibody titers after treatment, so as recognized an ideal diagnostic method. |
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