| 李新武,孙守宏,李涛,李晓青,李秋丽,袁洽劻.酸化电位水对微生物的杀灭效果及其作用机理的初步研究[J].Chinese journal of Epidemiology,1996,17(2):95-98 |
| 酸化电位水对微生物的杀灭效果及其作用机理的初步研究 |
| Preliminary Study on Microbiocide Effect and its Mechanism of Electrolyzed Oxidizing Water |
| Received:November 20, 1995 Revised:January 05, 1996 |
| DOI： |
| KeyWord: 酸化电位水 氧化还原电位 超微结构 |
| English Key Word: Electrolyzed oxidizing water Oxidizing reduation potential Ultrastructure |
| FundProject: |
| Author Name | Affiliation | | Li Xin-wu | Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing 102206 | | Sun Shou-hong | Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing 102206 | | Li Tao | Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing 102206 | | 李晓青 | Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing 102206 | | 李秋丽 | Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing 102206 | | 袁洽劻 | Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing 102206 |
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| Abstract: |
酸(氧)化电位水是一种具有高氧化还原电位(ORP)、低pH值和氧化能力的水,在无有机物存在的条件下,作用15秒钟可杀灭细菌繁殖体,作用30秒钟可破坏乙型肝炎表面抗原的抗原性,作用10分钟可杀灭枯草杆菌黑色变种芽孢。酸化电位水在室温密闭避光等条件下保存三个星期以上,其ORP值和pH值没有明显改变,蒸馏水、生理盐水对其影响较小,而有机物、磷酸盐等对其影响较大。
在电镜下观察酸化电位水对细菌芽孢的作用情况,结果表明:作用5分钟可使细菌细胞膜通透性增强、细胞肿胀、内容物溢出以及一些超微结构改变。 |
| English Abstract: |
Electrolyzed Oxidizing water (EO Water) is characterized by possessing higher oxidizing reduction potertial (ORP), lower pH value and oxidizing potential. Under conditions of free organic matter, it was tested for microbiocide efficacy in laboratory. The results showed that EO Water could completely kill all of the staphylococcus aureus and E. coli within 15 seconds, while for completely killing of spores of Bacillus subtilis Var. niger it would take 10 min. When it was used to destroy the antigenicity of HBsAg, 30 seconds was needed. The ORP and pH values of EO water were not obviously changed when stored in room-temperature with, airtight and light-free conditions for three weeks. Distilled water and physiological saline had little influence on the ORP and pH value of EO Water, but organic matters and phosphates. had greater influence upon the two values.
The ultrastructure of spores of Bacillus subtilis var. niger after exposure to EO Water was observed under transmission electronmicroscope and was compared with that of the non-disinfected spores. The results indecated that after exposure to EO Water for 5 min.,an increase of permeability of the spore's outer coat, swollen cell, overflowing of the contents of the spores and change of ultrastructure of spores were noticed. |
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