| 徐凌云,赵文彬,刁连东,吴桃林,张学年,周克捷,王理兴.环境水中脊髓灰质炎病毒二年循环动态研究[J].Chinese journal of Epidemiology,1996,17(4):236-238 |
| 环境水中脊髓灰质炎病毒二年循环动态研究 |
| A Study of the Cyclic Development of Poliomylitic Virus in Pond Foul Water during A Two-Year Span |
| Received:January 05, 1996 Revised:March 28, 1996 |
| DOI： |
| KeyWord: 脊髓灰质炎病毒 疫苗株 |
| English Key Word: Polio virus Strain of sabin |
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| Abstract: |
| 1993~1995年作者在连云港市选近三年曾有脊髓灰质炎病例的乡(镇),采用氯化钠-三氯化铝沉淀法对河塘污水中脊髓灰质炎病毒(PV)循环动态进行研究。发现:在连续二年的各个季节采集的水样中均能分离到致Hep-2细胞病变的病毒(57.95%),经鉴定:21株为PV(23.86%),其中PV Ⅰ 18株、PV Ⅱ 1株、PV Ⅲ 2株,另30株(34.09%)为非脊灰肠道病毒(NPEV)。鉴于我国目前脊灰流行毒株主要为PV Ⅰ,经RT-PCR试验,所分离的PV Ⅰ均为疫苗(Sabin)相关株,初步表明调查地区的外环境的PV已被疫苗相关株所取代。 |
| English Abstract: |
| A study on polio virus in pond foul water of town and counties in Lian Yun Gang city which had a reported case of polio. Foul water was examined by 1% NaCl-AlCl3 precipitated method quarterly during 1993-1995, to observe the cyclic development of the virus. Results showed that the isolation rate of polio virus was 57.95% with 21 strains of 18 PV Ⅰ, 1 PV Ⅱ and 2 PV Ⅲ and 30 strains of NPEV (34.09%). In view of PV Ⅰ as main epidemic strain in our coun try, and related strains to sabin was confirmed using RT-PCR. The above data suggested that polio virus in the pond water had been replaced by relative strains of sabin. |
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