| 李允文,邵一鸣,罗小光,苏玲,张流波,陈均,方越,袁健中,张艺,陈惠萍,Josef Kostler,Hans Wolf.湖北省HIV-1流行毒株的基因序列测定和亚型分析[J].Chinese journal of Epidemiology,1997,18(4):217-219 |
| 湖北省HIV-1流行毒株的基因序列测定和亚型分析 |
| Subtype and Sequence Analysis of the C2-V3 Region of gp120 Genes among HIV-1 Strains in Hubei Province |
| Received:March 03, 1997 Revised:April 07, 1997 |
| DOI： |
| KeyWord: HIV-1 亚型 基因变异 序列测定 |
| English Key Word: HIV-1 Subtype Gene variation Sequencing |
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| Abstract: |
| 1996年8月下旬采集20份湖北省HIV-1感染者外周血，分离单个核细胞（PBMCs）提取核酸使用PCR进行扩增获得HIV-1膜蛋白（env）基因的核酸片段，并对其C2-V3及邻区350~450个核苷酸序列进行了测定和分析。研究结果表明，这20份样品均为B亚型的HIV-1毒株序列，彼此间的基因离散率为1.9%。与国际A-E亚型参考序列及部分地区B亚型代表株序列相比较，湖北的20个毒株与流行与我国云南德宏、四川和河南等地的B亚型毒株序列十分接近，均属于泰国B亚型；它们的基因离散率在1.9%~3.2%的范围内。根据以上数据及其它资料提示，HIV-1在湖北的流行时间不长，该流行区毒株的传入与流行在云南德宏州的相同亚型HIV-1毒株密切相关。 |
| English Abstract: |
| Twenty DNA fragments of HIV-1 env gene were amplified by PCR from uncultured peripheral blood mononuclear cells (PBMCs) obtained from HIV-1 sero positive people from Hubei province in the end of 1996.The C2-V3 region (about 450 bp) was sequenced.Sequence analyses showed that all of them were HIV-1 subtypes,B strain,with 1.9% gene divergence innergroup.The 20 subtype B strains,compared with sequences of several reference HIV-1 strains,were closely related to those found in Thailand,Myanma and Yunnan and other provinces in China.The nucleotide sequence divergence between them ranged from 1.9% to 3.2%. These results suggested that recent epidemic of B subtype HIV-1 strains in Hubei province and the responsible virus were very close to those identified in Yunnan province. |
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