| 李伯安,程云,王利平,李靖,高蓉,赵景民,王松山,张敏,罗清华.应用原位杂交技术检测人肝组织中输血传播病毒核酸[J].Chinese journal of Epidemiology,1999,20(5):303-305 |
| 应用原位杂交技术检测人肝组织中输血传播病毒核酸 |
| Detection of TTV DNA in liver tissue by in situ hybridization |
| Received:April 15, 1999 Revised:May 10, 1999 |
| DOI： |
| KeyWord: 输血传播病毒 肝组织 原位杂交 |
| English Key Word: TTV Liver tissiues In situ hybrdization |
| FundProject: |
| Author Name | Affiliation | | LI Bo-an | 解放军第三○二医院免疫研究室,北京,100039 | | CHENG Yun | 解放军第三○二医院免疫研究室,北京,100039 | | WANG Li-ping | 四川省攀枝花市职工总医院传染科 | | LI Jing | 解放军第三○二医院免疫研究室,北京,100039 | | GAO Rong | 解放军第三○二医院免疫研究室,北京,100039 | | ZHAO Jing-min | 解放军第三○二医院免疫研究室,北京,100039 | | WANG Song-shan | 解放军第三○二医院免疫研究室,北京,100039 | | ZHNAG Min | 解放军第三○二医院免疫研究室,北京,100039 | | LUO Qing-hua | 解放军第三○二医院免疫研究室,北京,100039 |
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| Abstract: |
| 目的：探讨输血传播病毒 (transfusiontransmittedvirus，TTV)在人肝组织中的分布状况及其意义。方法：采用地高辛标记探针,对肝组织中的TTV基因进行原位杂交定位检测。结果：原位杂交染色阳性者为细胞核团块状。 5例正常人中有 1例阳性,5例乙型肝炎患者有 3例阳性,4例丙型肝炎患者有 2例阳性,3例戊型肝炎患者有 1例阳性。结论：TTV可感染肝细胞并在肝细胞核内复制。正常人及不同型肝炎病人的肝细胞内均可感染TTV。 |
| English Abstract: |
| Objective：To probe the distribution of transfusion transmitted virus(TTV)in liver tissiue.Methods：TTV gene was detected in liver tissiue by situ-hybridization using digoxiginin-labeled probes.Results：TTV genes detection by in situ-hybridization are massing in nucleoli of liver cells.It was detected in liver tissiue from 1 of 5 healthy individuals,3 out of 5 patients with hepatitis B,2 out of 4 patients with hepatitis C,1 out of 3 patients with hepatitis E.Conclusion：The data suggested that TTV infection is existed in general population and in patients with different types of hepatitis.The virus can replicate in nucleolus of liver cells. |
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