| 时曼华,梁中兴,杜雪飞,蒋秀高,聂一新.中国七日热型、澳洲型钩端螺旋体分子流行病学调查[J].Chinese journal of Epidemiology,2000,21(2):91-93 |
| 中国七日热型、澳洲型钩端螺旋体分子流行病学调查 |
| Amolecular epidemiological investigation on Leptospira interrogans serovar hebdomadis and australia inChina |
| Received:October 28, 1999 |
| DOI： |
| KeyWord: 钩端螺旋体病 传染源 染色体 核糖体核糖核酸基因 |
| English Key Word: Leptospirosis Source of infection Chromosome rRNA gene |
| FundProject: |
| Author Name | Affiliation | | SHI Manhua | Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing 102206, China | | LIANG Zhongxing | Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing 102206, China | | DU Xuefei | 南京市卫生防疫站 | | 蒋秀高 | Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing 102206, China | | 聂一新 | Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing 102206, China |
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| Abstract: |
| 目的 确定耕牛是否为七日热型、澳洲型钩端螺旋体病的传染源. 方法 自钩端螺旋体病患者血液及耕牛尿中分离出的12株七日热型、澳洲型钩端螺旋体野生株染色体DNA用限制性内切酶EcoRⅠ酶切后, 进行染色体DNA限制性内切酶图谱及16S+23SrRNA基因限制性内切酶片段长度多态性分析. 结果 自钩端螺旋体病患者血液及耕牛尿中分离的同一血清型的钩端螺旋体野生株的染色体DNA酶谱及核糖核酸型相同. 血清型不同的钩端螺旋体野生株的染色体DNA酶谱及核糖核酸型则不相同. 结论 耕牛是我国七日热型及澳洲型钩端螺旋体病的传染源, 这对控制我国七日热型及澳洲型钩端螺旋体病的流行有着非常重要的现实意义. |
| English Abstract: |
| Objective In order to confirm whether cattle serve the source of infection for patients with Leptospira interrogans serovar hebdomadis and australia. Methods 12 field strains of leptospira interrogans serovar hebdomadis and ustralia were isolated from blood samples of patients with leptospirosis and farm cattle urine. An analysis of chromosome DNA restriction endonuclease patterns(REP)and restriction fragments length polymorphism(RFLP)of 16S+23S rRNA gene were processed by digestion of chromosome DNA using EcoR I. Results The same serovar field strains of Leptospira interrogans from blood samples of patients with leptospirosis and farm cattle urine resulted in unique restriction endo nuclease patterns(REP)and ribosomal types(RT). The chromosome DNAs from field strains that belonged to different serovars of Leptospira interrogans caused different restriction endonuclease patterns and ribosomal types. Conclusion In accordance with this Results, we recognize that farm cattle serve the infectious source of leptospirosis serovar hebdomadis and australia thus may have realistic significance in the control of leptospirosis serovar epidemic of hebdomadis and australia in China. |
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