| 常丙功,张健之,田小东,毕德增,蔡军,张远富,陆振豸.斑点热群立克次体HN-98株的分离和初步鉴定[J].Chinese journal of Epidemiology,2000,21(3):202-204 |
| 斑点热群立克次体HN-98株的分离和初步鉴定 |
| Isolation and identification of HN-98 strain of spotted fever group rickettsiae |
| Received:November 23, 1999 |
| DOI： |
| KeyWord: 斑点热群立克次体 分子流行病学 |
| English Key Word: Spotted fever group rickettsiae Molecular epidemiology |
| FundProject:卫生部科学研究基金（编号：96-10-80） |
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| Abstract: |
| 目的 从海南省斑点热疫源地不明热病人体内分离斑点热群立克次体。方法 用鸡胚卵黄囊培养法直接从斑点热疫区一不明热病人血标本中分离立克次体，继之用分子生物学及血清学试验对分离株进行鉴定。结果 从海南省琼中县斑点热疫区一不明热男性民工的血标本中分离到了一株立克次体，命名为HN 98株立克次体，西伯利亚种。结论 该结果进一步从病原学上证实海南省琼中县存在有斑点热疫源地。 |
| English Abstract: |
| Objective One strain of rickettsiae was isolated from a patient with unknown fever from Qiongzhong county in Hainan Provinve in 1998, using embryo nated hen eggs and proved to be the membe of rickettsiae by theMethods of mo rphology.It w as named HS-98 strain after the name o f the place and the year it w as iso lated.Methods The isolate was identified byMethods of Microcomplement fixation assay and OCR/RFLP and compared with know n species and strains of SFGR by DNA polymerase chain reactio n and
DNA polymerase chainreaction and was followed by restrictio n endonuckease fragment length polymorphism analysis.ResultsResults demonstrated that HN-98 strain was antigenically and geno ty pically identical to R.sibirica 246.Conclusion TheResults sug gested that there ex isted na tural foci of spotted fever in Qiongzhong Area of Hainan Pro vince. |
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