福氏2a志贺氏菌肠毒素基因分型

白石山; 李秀霞; 夏桂枝; 苑宏; 郑文艳; 王红

Abstract

白石山,李秀霞,夏桂枝,苑宏,郑文艳,王红.福氏2a志贺氏菌肠毒素基因分型[J].Chinese journal of Epidemiology,2000,21(4):280-282

福氏2a志贺氏菌肠毒素基因分型

Gena types of Shigella enterotoxins of S.flexneri 2a

Received:November 26, 1999

DOI：

KeyWord: 福氏志贺氏菌肠毒素基因聚合酶链反应

English Key Word: Shigella flexneri Enterotoxins Gene Polymerase chain reaction (PCR)

FundProject:

Author Name	Affiliation
BAI Shishan	Department of Infectious diseases, The 253th Hospital of PLA, Hohhot, 010051, China
LI Xiuxia	内蒙古医学院病理教研室
XIA Guizhi	南京军区福州总医院儿科
Yuan Hong	北京军区卫生部
Zheng Wenyan	Department of Infectious diseases, The 253th Hospital of PLA, Hohhot, 010051, China
Wang Hong	第一军医大学流行病学教研室

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Abstract:

目的对福氏2a志贺氏菌（Shigella flexneri 2a,F2a）的志贺氏肠毒素1（Shigella enterotoxin 1，SET1）和志贺氏肠毒素2（Shigella enterotoxin 2，SET2）进行基因分型，提高菌痢暴发流行时同源性分析的水平。方法对一起暴发F2a菌痢在现场流行病学调查的基础上，用聚合酶链反应（PCR）方法，检测43株F2a菌的志贺氏肠毒素1基因（set 1）和志贺氏肠毒素2基因（set 2），进行基因分型和同源性鉴定。结果 43株F2a菌株分为3种基因型，即1株set 1（-）/set 2（+）；3株set 1（+）/set 2（-）；39株set 1（+）/set 2（+）。结论本次菌痢暴发是经污染食物传播，暴发事件中夹杂着散发病例，set 1/set 2基因分型对F2a菌痢暴发流行中的同源性鉴定具有重要作用。

English Abstract:

Objective To identify homogenous clones of S.flexneri 2a, using gene distribution polymorphism of Shigella enterotoxin 1(set 1) and Shigella enterotoxin 2(set 2) in a Shigellosis outbreak caused by S.flexneri 2a. Methods In addition to field epidemiological investigation，43 strains of S.flexneri 2a were determined by polymerase chain reaction（PCR）. Results The 43 strains (1 from food, 1 from a carrier, 41 from patients) were divided into 3 clones(set1+/set2+ for 39 strains, set1-/set2+ for 1,set1+/set2- for 3 strains). Conclusion This outbreak was caused by S.flexneri 2a. The route of transmission was contaminated-food borne. Some sporadic cases were noticed during the outbreak. The method used in this study was satisfactory to the identification of S.flexneri 2a.

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