CYP1A1、GSTM1基因多态性与食管癌遗传易感性的关系

邵根泽; 苏艳蓉; 黄革; 温博贵

Abstract

邵根泽,苏艳蓉,黄革,温博贵.CYP1A1、GSTM1基因多态性与食管癌遗传易感性的关系[J].Chinese journal of Epidemiology,2000,21(6):420-423

CYP1A1、GSTM1基因多态性与食管癌遗传易感性的关系

Relationship between CYP1A1, GSTM1 genetic polymorphisms and susceptibility to esophageal squamous cell carcinoma

Received:May 29, 2000

DOI：

KeyWord: 食管肿瘤细胞色素P450酶谷胱甘肽硫转移酶多态现象

English Key Word: Esophageal neoplasm Cy tochrome P450 Glutathione S-transferase Polymorphism

FundProject:广东省卫生厅青年科学基金资助项目(B1997077)

Author Name	Affiliation
SHAO Genze	Biochemistry Department, Shantou University Medical College, Guandong 515031, China
SU Yanrong	Biochemistry Department, Shantou University Medical College, Guandong 515031, China
HUANG Ge	Biochemistry Department, Shantou University Medical College, Guandong 515031, China
WEN Bogui	Biochemistry Department, Shantou University Medical College, Guandong 515031, China

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Abstract:

目的探讨CYP1A1、GSTM 1基因多态性与食管癌遗传易感性的可能关系。方法采用PCR法对正常人和食管癌患者基因组DNA进行CYP1A1、GSTM 1基因分型。结果食管癌组GSTM1(- /- )型频率 (6 4% )高于正常对照组 (5 0 % ) (P<0.0 5 ) ;食管癌CYP1A1基因I/I、I/V、V/V基因型频率与正常组相比差异无显著性,尽管食管癌CYP1A1基因I/V、V/V型频率 (6 0 % )稍高于正常对照组 (5 0 % ) (P =0.16 )。当研究对象按CYP1A1(I/I)和GSTM 1基因分类时,发现 :①在GSTM1(+ )人群中,食管癌组CYP1A1(I/V、V/V)基因型频率 (6 4% )高于正常组 (4 1% ) (P<0.0 5 )。②在CYP1A1(I/I)型人群中,食管癌组GSTM 1(- /- )型频率 (6 7% )显著高于正常对照组 (4 0 % )(P <0.0 1)。结论 GSTM 1、CYP1A1基因多态性与食管癌易感性相关,GSTM1(- /- )型个体食管癌易感性增高；GSTM 1(+ ) /CYP1A1(I/V、V/V)型和GSTM 1(- /- ) /CYP1A1(I/I)型个体比GSTM1(+ ) /CYP1A1(I/I)基因型个体具有更高的食管癌的易感性

English Abstract:

Objective To investig ate the association betw een susceptibility of esophageal squamous cell carcinoma and the genetic polymorphisms of CYP1A1 and GSTM1.Methods Subjects were comprised of 107 eso phag eal cancer patients and 111 healthy controls.Genotyping of both CYP1A1 and GSTM1 were perfo rmed in cancer tissues of all 107 patients and peripheral blood leuko cy tes taken from the controls by polymerase chain reaction.Results　There were no significant differences in the frequency distribution ofCYP1A1 polymo rphisms betw een eso phag eal cancer patients and healthy controls although the frequency of CYP1A1 with at least one allele of Val showed slightly higher in individuals with esophageal cancer.However, significant difference was observed in the frequency of GSTM1-nulled individuals with esophageal cancer comparing with the co ntrols (P < 0.05).When subjects were catego rized by bo th CYP1A1 genotype and GSTM1 g enotype, GSTM1(-)became markedly expressed in patients with CYP1A1(I/ I)than in the corresponding controls (67 % versus 40 %, P <0.01).The frequency of CYP1A1 genotype with at least o ne allele o f Val (I/V and V/ V)w as also statistically higher in patients w ith GSTM1(+),comparing to the co rresponding controls (64 % versus 41%, P <0.05).Conclusions It was suggested that :gene tic polymorphisms of CYP1A1 and GSTM1 were susceptible to esophageal cancer ；individuals who are GSTM1-null have an increased risk of developing esophageal cancer ;individuals with combined CYP1A1(I/ I)and GSTM1(-)or with combined CYP1A1(I/ V, V/ V)and GSTM1(+)were more susceptible, when comparing to those with combined CYP1A1(I/I)and GSTM1(+).

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