| 朱建琼,彭志强,雷满根,王红,郑少敏,李润深,陈清,叶临湘.2000年东莞市麻疹病毒流行株基因型分析[J].Chinese journal of Epidemiology,2001,22(5):355-358 |
| 2000年东莞市麻疹病毒流行株基因型分析 |
| Analysis on the genotype of measles virus epidemic strains in Don~uan city, Guangdong province in 2000 |
| Received:January 30, 2001 |
| DOI： |
| KeyWord: 麻疹病毒 核糖核酸 基因型 |
| English Key Word: Measles virus Ribonucleic acid Genotype |
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| Abstract: |
| 目的了解东莞市麻疹病毒(measlesvirus,MV)流行株的基因型。方法2000年4～7月间东莞市发生有55例患者的麻疹流行,采用逆转录聚合酶链反应(RTPCR)方法直接检测麻疹病人急性期唾液中的麻疹病毒核糖核酸(RNA)的N基因和M基因,对任意选取的5份RTPCR阳性标本和疫苗株沪191的N基因扩增片段进行核酸序列测定,并进行序列系统树分析。结果用RTPCR方法直接检测的阳性率为87.3%;用血清抗MVIgMELISA方法检测的阳性率为70.9%,抗MVIgM阴性的病人唾液中有68.8%的标本MVRNA阳性。序列分析结果表明此流行株的基因型与世界麻疹病毒流行株不同,差异为1.4%～11.7%,可归属于H基因型。结论2000年东莞市麻疹病毒流行株核酸序列完全相同,为H基因型,说明本次流行的麻疹病毒具有同源性,来源于同一传染源。 |
| English Abstract: |
| Objective To analyze the genotype of the measles virus epidemic strains in Dongguan according to the patients suffered measles in 2000. Methods The saliva of 55 patients with acute measles symptoms were collected and detected with RT PCR. The PCR products of the 5 samples were sequenced and analyzed with the software DNASTAR. Results The positive detection rate was 87.3 %, whereas the positive rate of anti MV IgM in serum was only 70.9 %. 68.8 % of the saliva samples of patients with anti MV IgM negative results showing MV RNA positive.The 293 bp fragment of N gene and 194 bp fragment of M gene were amplified from the vaccine virus and the specimens. The sequences of the epidemic strains were identical in both M gene and N gene. Conclusion These results suggested that the outbreak came from a single MV strain. The epidemic strain was obviously different from the standard strains discovered in other countries of the world (the deviation were 1.4 %～ 11.7 %) that might belong to H genotype. |
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