| 倪大新,汪华,顾玲,郭喜玲,庄菱,施平,潘浩,史智扬,胡晓抒,刘光中.江苏省1999年大肠埃希菌O157：H7宿主动物带菌情况调查[J].Chinese journal of Epidemiology,2002,23(2):102-104 |
| 江苏省1999年大肠埃希菌O157：H7宿主动物带菌情况调查 |
| Surveillance of Escherichia coli O157：H7 among animals in Jiangsu province in 1999 |
| Received:November 10, 2001 Revised:June 18, 2012 |
| DOI： |
| KeyWord: 大肠杆菌O157：H7 宿主动物 带菌率 毒力基因 |
| English Key Word: Escherichia coli O157：H7 Host animal Carrier rate Toxic gene |
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| Author Name | Affiliation | | NI Daxin | 江苏省疾病预防控制中心急性传染病防制科, 南京 210009 | | WANG Hua | 江苏省疾病预防控制中心急性传染病防制科, 南京 210009 | | GU Ling | 江苏省疾病预防控制中心急性传染病防制科, 南京 210009 | | GUO Xiling | 江苏省疾病预防控制中心急性传染病防制科, 南京 210009 | | ZHUANG Ling | 江苏省疾病预防控制中心急性传染病防制科, 南京 210009 | | SHI Ping | 江苏省疾病预防控制中心急性传染病防制科, 南京 210009 | | PAN Hao | 江苏省疾病预防控制中心急性传染病防制科, 南京 210009 | | SHI Zhiyang | 江苏省疾病预防控制中心急性传染病防制科, 南京 210009 | | HU Xiaoshu | 江苏省疾病预防控制中心急性传染病防制科, 南京 210009 | | LIU Guangzhong | 江苏省疾病预防控制中心急性传染病防制科, 南京 210009 |
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| Abstract: |
| 目的 了解江苏省不同地区大肠埃希菌O15 7：H7宿主动物带菌情况及其毒力基因阳性率。方法 在不同流行强度的地区分别设立监测点,采集猪、鸡、羊、牛等家畜家禽粪便标本,用免疫磁珠法进行病原菌分离培养,并用多重引物聚合酶链反应进行毒力基因分析。结果 6个监测点共采集猪、鸡、羊、牛等家畜家禽粪便标本 176 7份,共检出大肠埃希菌O15 7：H7170株,总带菌率为 9.6 2 %。其中,以牛、羊带菌率较高,分别为 19.0 5 %和 12.0 1%。对 85株菌进行SLT1、SLT2、eaeA和hly 4种毒力基因的检测,5 6.4 7%的菌株毒力基因阳性,且以同时带有SLT2、eaeA和hly 3种毒力基因最为常见,占带毒菌株的 79.17%。结论 宿主动物带菌率与当地疾病流行强度有关,即有确诊病人的地区宿主动物带菌率及菌株毒力基因阳性率最高,其次为仅有零星病例的地区,而无相关病例的地区最低。提示加强宿主动物大肠埃希菌O15 7：H7监测,对疫情的分析和疫情预测具有重要意义。 |
| English Abstract: |
| Objective To understand the Escherichia coli O157：H7 carrier rate of host animals and the toxic gene of the strains in different areas in Jiangsu province. Methods Surveillance spots were set up in different areas, to collect feces of pigs, chickens, sheep, cattle to culture for O157：H7 with immunomagnetic separation as well as detection of toxic gene of the strain with MPCR were both carried out. Results One hundred and seventy strains of O157：H7 were separated from 1 767 feces of different animals in six spots,with a overall positive rate 9.62 %. The positive rates of cattle and sheep were 19.05 % and 12.01% respectively. Among 85 strains SLT1,SLT2, eaeA and hly toxic genes were detected. In which, 56.47 % of the strains were positive curturely while 79.17 % of them carried SLT2, eaeA and hly gene simultaneously. Conclusion The positive rate of O157：H7 in animals and the positive rates of strains were correlated to the incidence of the area. The highest rates were seen in areas where there had been O157：H7 epidemic, followed by the areas where there were only scattered cases identified while the lowest was in areas with no patients. Data indicated that it was important to enforce the surveillance of O157：H7 in animals to better predict and control of the disease. |
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