| 姜波,秦杰,车立珍.甘南县四起家庭成员患肾综合征出血热的特点分析[J].Chinese journal of Epidemiology,2002,23(2):113 |
| 甘南县四起家庭成员患肾综合征出血热的特点分析 |
| Genetic characterization of coxsackievirus A16 isolated in Ningxia Hui Municipality in 2008 |
| Received:November 29, 2001 Revised:June 26, 2012 |
| DOI： |
| KeyWord: 手足口病 柯萨奇病毒A组16型 B1基因亚型 进化分支 |
| English Key Word: Hand, foot, and mouth disease Coxsackievirus A16 B1 subgenotype Evolution branch |
| FundProject:国家自然科学基金(30900063);"十一五"国家科技支撑计划(2008BAI56B00) |
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| Abstract: |
| 1994～2000年我县共发生肾综合征出血热病例1 388例,在进行个案流行病学调查中发现,有四起属家庭成员患病,共计9例,现将其特点分析如下. |
| English Abstract: |
| Objective To study the genetic characterization of coxsackievirus A16 (CVA16)strains isolated during an epidemic of hand, foot, and mouth disease (HFMD) in Ningxia Hui Municipality in 2008. Methods Clinical samples were collected from HFMD patients in Ningxia Hui Municipality and CVA16 strains were isolated by viral isolation methods. Reverse transcriptionpolymerase chain reactions (RT PCR), specific for CVA16 were performed with these CVA16 strains.Entire VP1 coding region amplification and sequencing were then performed and finally phylogenetic tree was constructed among Ningxia CVA16 strains and CVA16 representative strains of known genotypes and subgenotypes.Results 70 Ningxia CVA16 strains were isolated from HFMD patients in Ningxia in 2008 and the homology of nucleotide and amino acid were 90.8% 100.0% and 98.9% 100.0%, respectively. Phylogenetic characteristics of the strains reconfirmed that they could be divided into two distinct genotypes A and B. Genotype B could be further divided into the subgenotypes B1 and B2, while all the 70 Ningxia CVA16 strains belonged to the co circulated clusters B1a and Blb within subgenotype B1, which belonged to 2 viral transmission chainsb Our results indicated that subgenotype B1 CVA16 strains continued to circulate over a wide geographic area of mainland China since the first reported episode in Shenzhen city in 1999. Like other CVA16 strains isolated elsewhere in China, both Bla and Blb evolution branches were co circulating in Ningxia Hui Municipality. Based on the close phylgenetic and chronological relationship with CVA16 isolated in other countries and regions near China. Our data confimed that these strains co evolved and co circulated with those from neighboring countries and regions. |
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